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A kind of pH-responsive reversible fluorescent probe and preparation method thereof

A fluorescent probe, fluorescence technology, applied in fluorescence/phosphorescence, material analysis by optical means, material excitation analysis, etc., can solve the problems of liquid acid can not be loaded, limit the application range of basic fluorescent molecules, etc., to achieve simple raw materials Composition, increased utilization efficiency, effect of simple synthesis method

Active Publication Date: 2019-04-23
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liquid acid can be used to provide a proton environment to quench fluorescence, but liquid acid cannot be loaded on the carrier, which limits the application range of basic fluorescent molecules

Method used

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  • A kind of pH-responsive reversible fluorescent probe and preparation method thereof
  • A kind of pH-responsive reversible fluorescent probe and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0022] A pH-responsive reversible fluorescent probe is prepared from the following raw materials in parts by weight: 0.01 part of a fluorescent molecule (fluorescein isothiocyanate), a quencher (H 3 PMo 12 O 40 ) 99.99 copies. Adding fluorescent molecules and quenchers to water, stirring to dissolve, and then removing water, a fluorescent probe that can respond sensitively to pH changes is obtained. Strong fluorescence, and its fluorescence intensity can change reversibly with acid and alkali.

Embodiment 2

[0024] A pH-responsive reversible fluorescent probe is prepared from the following raw materials in parts by weight: 0.2 part of a fluorescent molecule (2-naphthylamine), a quencher (H 4 SiW 12 O 40 ) 99.8 servings. Add fluorescent molecules and quenchers to ethanol, stir to dissolve, and then remove ethanol to obtain a fluorescent probe that can respond sensitively to pH changes. It has strong fluorescence, and its fluorescence intensity can change reversibly with acidity and alkalinity.

Embodiment 3

[0026] A pH-responsive reversible fluorescent probe is prepared from the following raw materials in parts by weight: 2 parts of a fluorescent molecule (dichlorofluorescein), 2 parts of a quencher (H 3 AsMo 12 O 40 ) 98 copies. The fluorescent molecule and quencher are added to methanol, stirred to dissolve, and then methanol is removed to obtain a fluorescent probe that can respond sensitively to pH changes. It has strong fluorescence, and its fluorescence intensity can change reversibly with acidity and alkalinity.

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Abstract

Belonging to the technical field of molecular imaging, the invention in particular relates to a pH responsive reversible fluorescent probe and a preparation method thereof. The pH responsive reversible fluorescent probe comprises, by weight, 0.01%-20% of a fluorescent molecule and 80%-99.99% of a solid acid quenching agent, and the fluorescent molecule can fluoresce under an alkaline condition. The invention adopts simple raw material composition and a simple synthesis method to prepare the fluorescent probe with sensitive response to pH change, and because of the adding of solid acid, the utilization efficiency of the fluorescent molecule in actual operation is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of molecular imaging, and in particular relates to a pH-responsive reversible fluorescent probe and a preparation method thereof. Background technique [0002] Fluorescent probes have characteristic fluorescence in the ultraviolet-visible-near-infrared, and their fluorescence properties (excitation and emission wavelengths, intensity, lifetime, polarization, etc.) can vary with the properties of the environment, such as polarity, refractive index, viscosity, etc. Change responsively. Since a fluorescent probe can fluoresce at the molecular level, its ability to fluoresce depends on the molecular structure of the fluorescent probe, and its fluorescence after intermolecular interactions with nucleic acids (DNA or RNA), proteins or other macromolecular structures The properties will change, so fluorescent probes have been widely used in single-cell imaging, studying the properties and behavior of macromolecule...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李国良王军鹏张绘齐涛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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