Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel modeling method of rat rheumatoid arthritis

A rheumatoid arthritis, mouse technology, applied in pharmaceutical formulations, preparations for in vivo testing, resistance to vector-borne diseases, etc., can solve problems such as inability to induce arthritis models

Pending Publication Date: 2017-05-31
长春长生生物科技有限责任公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Incomplete Freund's adjuvant is a water-in-oil emulsion used to prepare immunogens, which can assist the antigen to stimulate the body to produce an efficient and long-term antibody response, but using it alone to immunize rats cannot induce an ideal arthritis model

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1) Mix recombinant Borrelia burgdorferi P100 protein with a final concentration of 5 mg / mL, CGCpGGC oligonucleotide with a final concentration of 1 mg / mL and incomplete Freund's adjuvant using an ultrasonic cell disruptor to make whole protein Emulsion, incubate at 37°C for 20 minutes (min) before use;

[0045] The test group consisted of 18 Lewis rats. The prepared 0.1mL whole bacterial protein emulsion was intradermally injected in the right paw of the rats, and 2 weeks (w) later, 0.1mL whole bacterial protein emulsion was intraperitoneally injected to boost the immunization for 1 Second-rate. The control group was 18 Lewis rats, which were injected with 0.25mL, 0.85% NaCl in the same procedure;

[0046] On the 16th day after modeling, the success rate of RA modeling in the experimental group was 86.66%. Pathology of the diseased joints showed synovial hyperplasia, inflammatory cell infiltration, cartilage tissue and bone tissue erosion, showing typical arthritis les...

Embodiment 2

[0074] 1) Mix recombinant Borrelia burgdorferi P100 protein with a final concentration of 10 mg / mL, CGCpGGC oligonucleotide with a final concentration of 3 mg / mL and incomplete Freund's adjuvant using an ultrasonic cell disruptor to make whole protein Emulsion, incubate at 37°C for 20 minutes (min) before use;

[0075] The test group consisted of 18 Lewis rats. The prepared 0.1mL whole bacterial protein emulsion was intradermally injected in the right paw of the rats, and 2 weeks (w) later, 0.1mL whole bacterial protein emulsion was intraperitoneally injected to boost the immunization for 1 Second-rate. The control group was 18 Lewis rats, which were injected with 0.25mL, 0.85% NaCl in the same procedure;

[0076] On the 16th day after modeling, the success rate of RA modeling in the experimental group was 94.44%. The pathology of the diseased joints showed synovial hyperplasia, inflammatory cell infiltration, cartilage tissue and bone tissue erosion, showing typical arthriti...

Embodiment 3

[0104] 1) Mix recombinant Borrelia burgdorferi P100 protein with a final concentration of 5 mg / mL, CGCpGGC oligonucleotide with a final concentration of 1 mg / mL and incomplete Freund's adjuvant using an ultrasonic cell disruptor to make whole protein Emulsion, incubate at 37°C for 20 minutes (min) before use;

[0105] The test group consisted of 18 Lewis rats. The prepared 0.25mL whole bacterial protein emulsion was intradermally injected in the right foot of the rats, and 2 weeks (w) later, 0.25mL whole bacterial protein emulsion was intraperitoneally injected to boost the immunization 1 times; the control group was 18 Lewis rats, injected 0.25mL, 0.85% NaCl with the same procedure;

[0106]On the 16th day after modeling, the success rate of RA modeling in the experimental group was 94.44%. The pathology of the diseased joints showed synovial hyperplasia, inflammatory cell infiltration, cartilage tissue and bone tissue erosion, showing typical arthritis lesions; The mice did...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention achieves the purpose by providing a novel modeling method of rat rheumatoid arthritis. On the basis of the conventional incomplete Freund's adjuvant, recombinant Borrelia burgdorferi P100 protein and CGCpGGC oligonucleotide are combined together for a modeling experiment of a rheumatoid animal model of a Lewis rat so as to establish a stable and high-efficiency novel modeling method. Due to the adoption of the modeling method, the modeling success rate is 88.89% or above, a foundation is laid for studying and developing a medicine for treating the rheumatoid arthritis, and the purpose of stably and efficiently preparing the rheumatoid arthritis animal model of the rat is achieved.

Description

technical field [0001] The invention provides a new rat rheumatoid arthritis modeling method and belongs to the field of biotechnology. Background technique [0002] Rheumatoid Arthritis (RA) is a chronic inflammatory autoimmune disease with an incidence of about 1% worldwide. The RA animal model can simulate the pathogenesis of RA, provide the pathology, genetics and molecular biology data of RA, and play an important role in the study of the pathogenesis and treatment strategies of RA. Therefore, animal models are widely used in RA research and new drug development. At present, there are many animal models used to study RA, including collagen type II induced arthritis (CIA), adjuvant-induced arthritis (AA), streptococcal cell wall-induced arthritis, prism Induces arthritis and proteoglycans induces arthritis, etc. These animal models all have defects in varying degrees, such as low stability of individual modeling, poor consistency between batches, and low success rate ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/02A61K49/00
CPCA61K39/0225A61K49/0008A61K2039/55561A61K2039/55566Y02A50/30
Inventor 王群王雷王超刘昶李莹王欣李强王玉莹吕品王美华
Owner 长春长生生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com