Method for detecting MEG3 gene SNP related to cattle growth traits and application thereof

A technology for growth traits and detection methods is applied in the field of detecting MEG3 gene SNPs related to growth and development traits of Chinese cattle breeds, which can solve the problems of growth trait effects and no reports, and achieve the effects of simple operation and low cost.

Active Publication Date: 2017-05-31
NORTHWEST A & F UNIV
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Problems solved by technology

In the study of livestock and poultry, multiple variant sites have been found to have significant effects on growth traits, but have not been reported in Chinese yellow cattle

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  • Method for detecting MEG3 gene SNP related to cattle growth traits and application thereof
  • Method for detecting MEG3 gene SNP related to cattle growth traits and application thereof
  • Method for detecting MEG3 gene SNP related to cattle growth traits and application thereof

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Embodiment Construction

[0026] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The embodiments are an explanation of the present invention, not a limitation.

[0027] In the embodiment of the present invention, Chr 21: 67377727-67395997, the candidate region of the bovine MEG3 gene is used as the candidate site:

[0028] (1) Using sequencing technology to detect the SNP variation of candidate sites in the cattle population, using PCR-RFLP and Tetra-primer ARMS-PCR technology to classify different SNP sites of different individuals.

[0029] (2) Use SPSS 20.0 software to analyze the association between SNP types and cattle growth traits.

[0030] (3) According to SNP type, select and breed cattle individuals with excellent growth traits.

[0031] 1. Scalper sample collection

[0032] The present invention specifically takes populations of 3 Chinese local cattle breeds, namely Shaanxi Qinchuan cattle (106), Henan Biyang County Xianan cat...

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Abstract

The invention discloses a method for detecting MEG3 gene SNP related to cattle growth traits and an application thereof. According to the position of a mononucleotide mutation site in an exon area of the cattle MEG3 gene, parting is carried out on a cattle individual by adopting the PCR-RFLP and Tetra-primer ARMS-PCR technology, and correlation analysis is carried out on a parting result and growth data. In the detection method disclosed by the invention, an SNP mark closely related to the cattle growth traits is detected on the DNA level, and the detection method can be used in the marker assisted selection of cattle variety growth traits in China so as to accelerate the establishment of high-quality cattle population.

Description

Technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a method and application for detecting the MEG3 gene SNP related to the growth and development traits of Chinese cattle breeds. Background technique [0002] Single nucleotide polymorphisms (SNPs) refer to DNA sequence polymorphisms at the genome level caused by a single nucleotide change. A SNP has a single nucleotide change at a certain position in the genome. Most of it is caused by two situations: one is caused by a single base conversion, that is, one pyrimidine is replaced by another pyrimidine, or one One kind of purine is replaced by another kind of purine; the other kind of situation is transversion, that is, the exchange of purine and pyrimidine. SNPs can also be divided into protein-coding SNPs and non-protein-coding SNPs according to their effects on biological genetic traits. The protein-coding SNPs are located in the transcribed sequence and the non-...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/683C12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2535/137C12Q2521/301
Inventor 陈宏李波刘梅白跃宇蓝贤勇黄永震雷初朝
Owner NORTHWEST A & F UNIV
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