Fluorescence labeling composite amplification kit for 29 STR (short tandem repeat) gene loci of human Y chromosome

Abstract

The invention discloses a fluorescence labeling composite amplification kit for 29 STR (short tandem repeat) gene loci of human Y chromosome. The kit is characterized by comprising specific amplification primers of 29 pairs of STR gene loci, wherein the STR gene loci are DYS389I/II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438, DYS448, DYS456, DYS390, DYS449, DYS576, DYS481, DYS549, DYS533, DYS570, DYS643, DYS460, DYS627, DYS387a/b and DYS518. The kit has the advantages of high accumulated individual identification rate, capability of effectively reducing risk of allele crossing the border and the like.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a fluorescent labeling composite amplification kit for 29 STR loci of human Y chromosome. Background technique [0002] Short tandem repeat loci (Short Tandem Repeat, STR) genetic markers, also known as microsatellite DNA, are widely found in prokaryotic and eukaryotic genomes, and are a class of repeating units consisting of 2-6 nucleotides. Ten to more than 100 nucleotide repeat sequences, different numbers of core sequences are arranged in a central repeat, and the length is polymorphic. According to the conserved sequences at both ends, primers are designed, PCR is performed, and then polyacrylamide, Detect the polymorphism of STR genetic markers by agarose gel electrophoresis or capillary electrophoresis. Compared with other genetic markers, STR markers have small fragments and are easy to amplify. They are more suitable for trace and degraded samples. The amplification co...

AI Technical Summary

Problems solved by technology

[0005] At present, forensic medicine uses Y-STR to build a database. The main function is to check the family line and narrow down the scope of suspects, not to identify suspects. In China, especially in rural areas, it is mainly the way that families live together. The database built in Y-STR In actual operation, only one or several members of a large family are often sampled to build a library, so that the Y-STR can be used to quickly narrow the scope of investigation at a low cost, and then by comparing the autosomal STR, To identify the suspect, because one or several people sampled in the Y-STR database represent the Y-STR information of the whole family, there is a disadvantageous situation: the suspect belongs to a member of the family, but is not related to the established family. If there are 3 or more locus typing inconsistencies in the Y-STR of the library object, it is easy to exclude the real family to which the suspect belongs. If there are many rapid mutation loci in the library construction kit, this possibility will become Very high. On the other hand, since the main purpose of Y-STR database construction is not to identify suspects and does not need to distinguish related males, the advantage of rapid mutation loci is not very useful here. Therefore, it is not recommende...

Images