Fluorescence labeling composite amplification kit for 29 STR (short tandem repeat) gene loci of human Y chromosome

Abstract

The invention discloses a fluorescence labeling composite amplification kit for 29 STR (short tandem repeat) gene loci of human Y chromosome. The kit is characterized by comprising specific amplification primers of 29 pairs of STR gene loci, wherein the STR gene loci are DYS389I / II, DYS458, DYS19, DYS385a / b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438, DYS448, DYS456, DYS390, DYS449, DYS576, DYS481, DYS549, DYS533, DYS570, DYS643, DYS460, DYS627, DYS387a / b and DYS518. The kit has the advantages of high accumulated individual identification rate, capability of effectively reducing risk of allele crossing the border and the like.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a fluorescent labeling composite amplification kit for 29 STR loci of human Y chromosome. Background technique [0002] Short tandem repeat loci (Short Tandem Repeat, STR) genetic markers, also known as microsatellite DNA, are widely found in prokaryotic and eukaryotic genomes, and are a class of repeating units consisting of 2-6 nucleotides. Ten to more than 100 nucleotide repeat sequences, different numbers of core sequences are arranged in a central repeat, and the length is polymorphic. According to the conserved sequences at both ends, primers are designed, PCR is performed, and then polyacrylamide, Detect the polymorphism of STR genetic markers by agarose gel electrophoresis or capillary electrophoresis. Compared with other genetic markers, STR markers have small fragments and are easy to amplify. They are more suitable for trace and degraded samples. The amplification co...

AI Technical Summary

Problems solved by technology

[0005] At present, forensic medicine uses Y-STR to build a database. The main function is to check the family line and narrow down the scope of suspects, not to identify suspects. In China, especially in rural areas, it is mainly the way that families live together. The database built in Y-STR In actual operation, only one or several members of a large family are often sampled to build a library, so that the Y-STR can be used to quickly narrow the scope of investigation at a low cost, and then by comparing the autosomal STR, To identify the suspect, because one or several people sampled in the Y-STR database represent the Y-STR information of the whole family, there is a disadvantageous situation: the suspect belongs to a member of the family, but is not related to the established family. If there are 3 or more locus typing inconsistencies in the Y-STR of the library object, it is easy to exclude the real family to which the suspect belongs. If there are many rapid mutation loci in the library construction kit, this possibility will become Very high. On the other hand, since the main purpose of Y-STR database construction is not to identify suspects and does not need to distinguish related males, the advantage of rapid mutation loci is not very useful here. Therefore, it is not recommended to use rapid mutation loci in this case. Mutation rate loci, however, low mutation rate loci tend to be less polymorphic, which is not conducive to the distinction between families, therefore, the number of loci needs to be large, and the polymorphism of loci should be high at the same time to ensure Y-STR reagent gold has a sufficiently high overall polymorphism

[0006] At present, the mainstream commercial kits on the market usually use five-color fluorescence detection technology, but with the expansion of the Y-STR library construction market, the existing Y-STR kits can no longer meet the current market demand, and the market urgently needs a Y-STR kits with higher individual rate and more detection loci are the inevitable trend of STR fluorescence detection kits

[0007] The mainstream kits on the market include Yfiler, Yfiler Plus, PowerPlexY23, Goldeneye Y26, STRtyper27Y, AGCU Database Y24 and other fluorescent detection kits, the number of loci are 17, 27, 23, 26, 27, 24, Yfiler, which is commonly used in forensic medicine, has a small number of loci and a low cumulative individual recognition rate. Yfiler has been confirmed to have very low polymorphisms in some specific populations. Yfiler P1us, STRtyper 27Y, powerplex Y23, and Goldeneye Y26 contain 7, 7, 2 and 3 rapidly mutating genes

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