Method for authenticating sugarcane Pokkah Boeng resistance with in-vitro leaves

Abstract

The invention discloses a method for authenticating sugarcane Pokkah Boeng resistance with in-vitro leaves. The method includes the following steps that 1, a PDA culture medium plate is inoculated with sugarcane tip fusarium moniliforme, and culture is conducted for 3-5 days at 25-28 DEG C; 2, fresh sugarcane leaves are collected, washed and cut into segments, the two sides of veins at the middle portions of the leaves are punctured, the punctured portions are inoculated with sugarcane tip fusarium moniliforme blocks, and the faces where hyphae grow are attached to the wounds; 3, the sugarcane leaves inoculated with the sugarcane tip fusarium moniliforme are placed in a culture dish, sterile water is sprayed, and the culture dish is placed for 3-5 days at the room temperature; 4, the infection condition is inspected, the length of scabs is observed and measured, the length of yellow halos is measured in the vein direction, and the Pokkah Boeng resistance of the sugarcane variety is evaluated according to the length of the scabs. According to the authenticating method, it is not needed to plant sugarcane seedlings according to seasons, the authenticating process is not limited by the age of sugarcane seedlings or the site, operation is easy and convenient, and repeatability is good; the authenticating method has the advantage of well saving time and labor for screening of a large number of samples.

Description

technical field [0001] The invention relates to a method for identifying sugarcane tip rot resistance by adopting isolated leaves. Background technique [0002] Sugarcane tip rot is an important leaf disease in sugarcane production. Sugarcane tip rot (Pokkah Boeng) is a disease of sugarcane leaves caused by a variety of Fusarium fungi. The pathogen is Gibberella fujikuroi complex), the asexual state includes at least 5 species of Fusarium such as Fuarium sacchri, F. proliferatum, F. verticillioides, F. subglutinan and F. andiyazi. Pathogens are mainly transmitted to the heart leaves of sugarcane through airflow and cause damage to the leaves, resulting in death of leaves and stunted plant growth. In severe cases, it can cause plant death, resulting in a 5%-20% reduction in sugarcane yield and a 3% reduction in sugarcane sugar content. The impact is more pronounced. The disease is common in almost all sugarcane producing countries and regions, although the prevention and co...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is an evaluation of the resistance of sugarcane seedlings to tip rot by manual acupuncture inoculation at the seedling stage, but the whole process needs to undergo disinfection and sowing of sugarcane seeds, and the inoculation can only be done after the cane seedlings have grown to 3 to 4 complete leaves. It takes 10-15 days to observe and judge the disease resistance of sugarcane varieties

The whole cycle is long, and it takes at least 1.5 to 2 months for sugarcane to reach the appropriate seedling age for inoculation, and it is not easy to control the temperature and humidity in the greenhouse. Sowing in different seasons, the resistance of sugarcane is different, and it is easy to cause repeated test results. inconsistent

In addition, if the inoculation is not carried out in time, the resistance of the variety itself will continue to increase with the increase of seedling age, and the inoculation is difficult to cause disease of sugarcane leaves, resulting in the discrimination error of the resistance level

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