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Method for measuring virulence of pathogenic bacteria of pokkah boeng

A technology for sugarcane tip rot and pathogenicity, applied in the field of determining the pathogenicity of sugarcane tip rot pathogens, can solve problems such as sugarcane leaf rot, sugarcane variety yield and sugar content decline, plant death, etc., to ensure infection , easy measurement conditions, and effective repeatability

Inactive Publication Date: 2017-08-04
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] Sugarcane tip rot is an important fungal disease in sugarcane production. It mainly infects sugarcane shoots, causes sugarcane leaf rot, and even plant death, causing a significant decline in yield and sugar content of sugarcane varieties.

Method used

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  • Method for measuring virulence of pathogenic bacteria of pokkah boeng
  • Method for measuring virulence of pathogenic bacteria of pokkah boeng

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Embodiment

[0018] A method for measuring the pathogenicity of sugarcane tip rot pathogen, comprising the following steps:

[0019] 1. Preparation of inoculum

[0020] Fusarium, the pathogen of sugarcane tip rot, was cultured separately. Specifically: inoculate the pathogenic bacteria of sugarcane tip rot on a PDA medium plate to activate for 3 days, pick the edge mycelium and inoculate it on a new PDA medium plate, cultivate it at 25-28°C for 3-5 days, and use 1 mL of sterilized Dig the mycelium block (about 5-6mm in diameter) with the tip of the pipette (one end with a wide mouth), and set it aside.

[0021] 2. Treatment of inoculated materials

[0022] Select the sugarcane tip rot-susceptible variety GT37, the disease-resistant variety ROC22, and the moderately sensitive material GT08-120 in production, and collect fresh sugarcane leaves (the second to fourth fully expanded leaves from the heart leaf), and clean them with clean water. Rinse off the dust on the surface, dry it, and c...

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Abstract

The invention discloses a method for measuring virulence of pathogenic bacteria of pokkah boeng. The method comprises the following steps of (1) inoculating the pathogenic bacteria of the pokkah boeng onto a PDA (potato dextrose agar) culture medium flat plate, and culturing for 3 to 5 days at the temperature of 25 to 28 DEG C; (2) selecting a disease infecting variety of the pokkah boeng, collecting fresh sugarcane leaves, cleaning the leaves, shearing the leaves into sections, puncturing both sides of the middle vein of each leaf, inoculating the pathogenic bacteria mass of the pokkah boeng onto each puncturing part, and attaching one side with hypha to a wound; (3) putting the pathogenic bacteria-inoculated sugarcane leaf into a culture dish, spraying sterile water, and standing for 3 to 5 days at room temperature; (4) observing and measuring the length of the pathogenic bacteria spot, measuring the length of the yellow corona along the leaf vein, and using the length of the pathogenic bacteria spot to distinguish the strength and the weakness of virulence of pathogenic bacteria. The method for measuring the virulence of the pathogenic bacteria of the pokkah boeng has the advantages that the virulence of the pathogenic bacteria of the pokkah boeng is measured by the in-vitro inoculation way, the collecting of the sugarcane leaf sample is free from the influence by the sugarcane seedling age and planting period, and the measuring condition can be easily controlled.

Description

technical field [0001] The invention relates to a method for measuring the pathogenicity of sugarcane tip rot pathogen. Background technique [0002] Sugarcane tip rot is an important fungal disease in sugarcane production. It mainly infects sugarcane shoots, causes sugarcane leaf rot, and even plant death, causing a significant decline in yield and sugar content of sugarcane varieties. Sugarcane tip rot occurs widely in my country. Almost all sugarcane varieties can be infected with tip rot. The incidence of tip rot in susceptible varieties can reach more than 38%, resulting in a 5%-20% reduction in sugarcane production and a 3% reduction in sugarcane sugar content. . Sugarcane tip rot (Pokkah Boeng) is a disease of sugarcane leaves caused by a variety of Fusarium fungi. The pathogen is Gibberella fujikuroicomplex. The asexual form includes at least Fuarium sacchri, F. proliferatum, F. 5 species of Fusarium such as verticillioides, F.subglutinan and F.andiyazi. The applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/24C12R1/77
CPCC12Q1/04C12Q1/24
Inventor 林善海王泽平罗霆李毅杰黄诚华
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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