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Cleaning fluid for biological chip

A biochip and cleaning solution technology, applied in detergent compounding agent, detergent composition, organic cleaning composition, etc., can solve the problems of differences in detection results, high analysis signal-to-noise ratio, high background, etc.

Inactive Publication Date: 2017-06-20
TAIZHOU SYNO GENE DIGITAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of high background caused by insufficient washing of biochips in commonly used phosphate buffer solution in the prior art, which causes differences in detection results, and to provide a cleaning solution for biochips. The cleaning solution has the ability to make the chip membrane Advantages of low surface background signal and high signal-to-noise ratio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. First prepare 0.5M EDTA solution:

[0033] 1) Accurately weigh 46.53g of disodium edetate into a 500ml beaker;

[0034] 2) Add 200ml of deionized water to a 500ml beaker with a graduated cylinder, stir and dissolve with a magnetic heating stirrer;

[0035] 3) Add NaOH to adjust the pH value to 8.0, and set the volume to 250ml.

[0036] 2. Then prepare 1.25X TBE buffer:

[0037] 1) Accurately weigh 135g Tris and pour it into a 5000ml beaker;

[0038] 2) Accurately weigh 68.75 grams of boric acid and pour it into a 5000ml beaker;

[0039] 3) Add 2000ml deionized water into a 5000ml beaker with a measuring cylinder and mix well;

[0040] 4) Add 50ml of 0.5M disodium EDTA solution to the solution in step 3), and mix well;

[0041] 5) The solution in step 4) is poured into a 3000ml graduated cylinder to set the volume to 2500ml, then poured into a 5000ml beaker and mixed evenly.

[0042] 3. Preparation of cleaning solution for biochip

[0043] Prepared according to...

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PUM

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Abstract

The invention belongs to the technical field of biological chips, and particularly relates to cleaning fluid for the biological chip. The cleaning fluid is prepared from the following components in percentage by weight: 50 percent of a 1.25X TBE buffer solution, 10 percent of an ammonium sulfate solution, 2 percent of a nonionic surfactant, 0.02 percent of Procl in300 and the balance of deionized water. The cleaning fluid disclosed by the invention has the advantages of low background signal intensity on the film surface of the chip and high analysis signal-to-noise ratio.

Description

technical field [0001] The invention belongs to the technical field of biochips, in particular to a cleaning solution for biochips. Background technique [0002] At present, the commonly used washing solution is phosphate buffer saline (PBS) solution, and the formula is to weigh 8g NaCl, 0.2g KCl, 1.44g NaCl 2 HPO 4 and 0.24g KH 2 PO 4 , Dissolve in 800ml distilled water, adjust the pH value of the solution to 7.4 with HCl, and finally add distilled water to adjust the volume to 1L. [0003] Wash steps reduce background signal from unbound antibody, thereby increasing the signal-to-noise ratio of the assay. Washes between each step ensure that only specific binding events are retained, generating signal in the final step. Inadequate washes can lead to discrepancies and high background, resulting in suboptimal results. PBS solution is used as a washing solution, which will cause insufficient washing, often high background, and cause differences in test results. Conten...

Claims

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Application Information

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IPC IPC(8): C11D1/74C11D3/60C11D3/34C11D3/30C11D3/33C11D3/04
CPCC11D1/74C11D3/042C11D3/046C11D3/30C11D3/33C11D3/3481
Inventor 潘宝华
Owner TAIZHOU SYNO GENE DIGITAL TECH CO LTD