Primers for identifying soybean male sterile line and method thereof

Abstract

The invention relates to the technical field of gene engineering, and provides primers for identifying a soybean male sterile line and a method thereof. The primers are disclosed as SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: carrying out PCR (polymerase chain reaction) amplification and BstXI enzyme digestion on the template soybean genome DNA by using the primers; and judging the soybean is the male sterile line if the product only contains one 472bp strip, and judging the soybean is the male fertile line if the product comprises 266bp and 202bp. On the basis of detecting that the soybean male-sterile character is controlled by a pair of recessive single genes, the invention develops a codominant molecular marker for identifying the soybean male sterile line, and provides the primers for identifying the soybean male sterile line and the method thereof. The primers and method can perform efficient accurate identification on the soybean fertility and genotype in any period in the soybean breeding process, thereby providing a new way for molecular identification of the soybean male sterile line.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a primer for identifying soybean male sterile lines and a method thereof. Background technique [0002] Conventional hybridization is the most commonly used method in soybean breeding, but this method advocates selecting the best from the best, and one of the parents is often a local excellent variety. In the long run, the relatives of the varieties used in production will be very close, which will inevitably lead to a trend in the genetic basis. Therefore, it has been unable to achieve the high-standard breeding goals of high quality, high yield, multiple resistance and broad genetic basis. Therefore, people have gradually paid attention to the use of recurrent selection for population improvement to solve the problem of narrow genetic basis in conventional hybridization. [0003] As a self-pollinating crop, soybean has small flower organs, difficult hybridization a...

AI Technical Summary

Problems solved by technology

The second category is male infertility and female infertility. This type of infertility is difficult to apply in genetic breeding and has no practical significance

[0006] The identification method of soybean male sterility mainly relies on phenotypic identification at present, and it is difficult to accurately identify soybeans in the early growth stage

And if it can be identified at the molecular level, it is undoubtedly the most efficient and accurate method, but currently there are few effective patents for molecular marker technology for male sterility in soybeans, and the present invention is just the research achievement of this aspect

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