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Method for identification and evaluation of cold resistance of red beans

A technology for adzuki bean and cold resistance, which is applied in the field of identification and evaluation of the cold resistance of adzuki bean, and can solve the problems of nutrient depletion, withering, and abnormal leaf photosynthesis.

Active Publication Date: 2017-06-27
FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low temperature obstacle at the seedling stage of the red bean is due to the low temperature and low light, and the chlorophyll synthesis of the primary leaves is hindered, resulting in delayed leaf growth, resulting in abnormal photosynthesis of the leaves, resulting in nutrient depletion, and eventually they cannot recover and die

Method used

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  • Method for identification and evaluation of cold resistance of red beans
  • Method for identification and evaluation of cold resistance of red beans
  • Method for identification and evaluation of cold resistance of red beans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, by H 2 o 2 Identification of Cold Resistance of Adzuki Bean by Content Change

[0036] 1. The processing method of red bean

[0037] The most representative cold-resistant varieties at emergence in Hokkaido, Japan—Akagen Danagyan and the non-cold-resistant variety at emergence—Madara small grain line-1 were selected. The two varieties are respectively planted in a nutrient bowl, and vermiculite is packed into the nutrient bowl, and 30-40 red bean seeds are sown at intervals of 2 cm in each nutrient bowl, and are repeated three times. After sowing, place the seedlings at a constant temperature of 25°C and 100% darkness for emergence, and move to a light intensity of 700-1000 μmol / m after emergence. 2 / s, the red bean of each variety was divided into two groups, which were grown under low temperature conditions (10°C to 13°C day and night) for 18d and 28d respectively, and then each group was divided into three groups and moved to a natural light room at ...

Embodiment 2

[0048] Embodiment 2, identify the cold resistance of red bean by the change of chlorophyll content

[0049] 1. The processing method of red bean

[0050] Same as Step 1 of Example 1.

[0051] 2. Determination of chlorophyll content

[0052] Take the young leaves of the cold-resistant variety Akagen Danayan and the non-cold-resistant variety Banxiaoli-1, and measure the chlorophyll content of the leaves at the seedling stage of the greening treatment for 0h, 3h, 8h and 28h after the short-term low temperature treatment for 18 days and the long-term low temperature treatment for 28 days, respectively . The determination of chlorophyll content was carried out according to the method of Kato Rong (Photosynthesis Research Methods. Kyoritsu Publishing House, Tokyo, 1981, 40-41). Each treatment setting was replicated at least 3 times.

[0053] The measurement results are shown in Table 2.

[0054] Table 2 Chlorophyll content of red bean seedling stage leaves after greening treat...

Embodiment 3

[0061] Embodiment 3, the activity measurement of antioxidant enzyme

[0062] 1. The processing method of red bean

[0063] Same as Step 1 of Example 1.

[0064] 2. Determination of Antioxidant Enzyme Activity

[0065] Under stress conditions, free radicals produced in plants can cause degreasing and peroxidation of plasma membranes, destroying the structure and function of biofilms, and antioxidant enzymes in plants include superoxide dismutase (SOD), ascorbic acid peroxidation Catalase (APX) and catalase (CAT), etc., can eliminate the adverse effects of reactive oxygen species and peroxides to a certain extent, and maintain the stability of cell membranes.

[0066] SOD is a metalloenzyme commonly found in aerobic organisms. It cooperates with peroxidase and catalase to defend the cell membrane from active oxygen or other peroxide free radicals. SOD can catalyze the disproportionation of oxygen free radicals. The reaction produces hydrogen peroxide, which can be converted i...

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Abstract

The invention discloses a method for identification and evaluation of cold resistance of red beans. The method specifically includes the steps of A), sowing the red beans to be measured the red beans to the environment with the light intensity of 700-1000ummol / m<2> / s and the temperature of 10-13DEG C to grow for 28-30 days after seedling, and then moving plants to the environment with the light intensity of 1000-1500ummol / m<2> / s and the temperature of 20-25DEG C for greening; B), measuring changing conditions of H2O2 content, chlorophyll content, SOD (superoxide dismutase) enzymatic activity, APX (antithrombotic peroxidase) enzymatic activity or CAT enzymatic activity in the plants with passing of greening time to determining the strength of cold resistance of the red beans to be measured. By the arrangement, the method for identification and evaluation of cold resistance of the red beans is accurate and rapid, which is of great significance to utilization of variety resources, reasonable planning and regulation of planting locations and cold resistant breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying and evaluating the cold resistance of red bean. Background technique [0002] Low temperature is an important environmental factor affecting plant growth, development and biomass. Low temperature damage is a natural disaster that often occurs in agricultural production, seriously affecting the quality and yield of crops, and even causing large-scale death of crops. So far, there is no fundamental solution to low temperature injury. [0003] my country is the country with the largest adzuki bean cultivation area and the largest export volume. Adzuki bean is the main edible bean crop in my country, but it is the least cold-resistant and low-temperature sensitive crop compared with other beans, which leads to unstable production of adzuki bean in cold regions. The cold resistance of red bean varieties is the main factor that determines its planting and promotio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/00
CPCG01N25/00
Inventor 何宁冷春旭曹大为王雪杨曹良子孟英洛育孙世臣唐晓东李一丹崔秀辉卢环李柱刚王立志唐傲张喜娟董文军刘猷红姜连子张连菊
Owner FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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