Ghlmm gene that can significantly improve cotton disease resistance and its application

Abstract

The invention discloses a GhLMM gene capable of substantially improving disease resistance of cotton and application thereof, belonging to the field of application of biotechnology. The invention provides the nucleotide sequences of the GhLMM gene in the genomic subgroup A and the genomic subgroup D of a standard genetic system of allotrtraploid upland cotton, the ORF sequences of the GhLMM gene, and amino acid sequences coded by the GhLMM gene. The GhLMM gene provided by the invention is acquired by subjecting a mutant gene of a cotton lesion-mimic mutant discovered for the first time to genetic analysis, fine mapping and map-based cloning. The GhLMM gene codes 5-aminolevulinic acid anhydrase (ALAD) and is an important gene in the process of metabolism of a tetrapyrrole compound in cotton; and inactivation of the gene leads to accumulation of a substrate, i.e., 5-aminolevulinic acid (ALA), so hydrogen peroxide and SA are produced and plant disease-resistance signals are activated. The GhLMM gene exerts dose effect on equilibrium between plant growth and disease resistance of plants. Optimal equilibrium between growth adaptability and disease resistance of plants can be realized by regulating the expression of the GhLMM gene.

Description

technical field [0001] The present invention relates to a cotton GhLMM gene, which encodes 5-aminolevulinic acid dehydratase (ALAD). Using the lesion-like mutant Ghlmm discovered in upland cotton for the first time, the gene was obtained by map-based cloning technology. In the Ghlmm mutant, due to the GhLMM gene located in the D subgroup, a single nucleotide SNP mutation caused the premature termination of the coding region and loss of function. The detection of disease resistance showed that the resistance of the mutant to Verticillium dahliae was significantly improved. Further, the genome sequence, full-length ORF sequence and encoded amino acid sequence of the gene were obtained in the upland cotton genetic standard line TM-1A subgroup and D subgroup by PCR technology. In tetraploid cotton, the gene contains four copies, and the use of biotechnology to regulate the expression dosage of the gene in tetraploid cotton can improve cotton disease resistance, and belongs to th...

AI Technical Summary

Problems solved by technology

Fine mapping and map-based cloning found that Ghlmm is due to a single nucleotide mutation in the Gh_D05G2237 coding region, which caused the premature termination of the translated polypeptide, resulting in a loss of function

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