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Method for determining content of crude protein in lolium multiflorum

A technology of ryegrass multiflora and a determination method is applied in the field of determination of crude protein content of ryegrass multiflora, which can solve the problems of complicated operation, long time consumption and high cost, and achieve the effects of high detection precision, high detection efficiency and simple operation

Inactive Publication Date: 2017-06-30
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, traditional wet chemical methods are mainly used for the determination of forage grass CP in China. There are outstanding problems such as complicated operation, large error, high cost, long time consumption, and environmental pollution caused by toxic and harmful chemicals. ) or samples to screen out varieties (lines) or samples with high CP content, so finding out a method that can quickly measure CP content in Lolium multiflora is of great significance for breeding of Lolium multiflora and quality control of forage

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  • Method for determining content of crude protein in lolium multiflorum
  • Method for determining content of crude protein in lolium multiflorum
  • Method for determining content of crude protein in lolium multiflorum

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Embodiment

[0052] A, establish ryegrass multiflora crude protein content near-infrared prediction model; described ryegrass multiflora crude protein content near-infrared prediction model adopts following method to establish, specifically comprises the following steps:

[0053] a. Collecting ryegrass multiflora samples, the ryegrass multiflora samples are the ryegrass multiflora samples collected in 2015-2016, including 16 national examination varieties and 22 new lines, and the collection growth period includes tillering stage and jointing stage , booting stage, heading stage, flowering stage, fruiting stage, and maturity stage. The collection parts include stems, leaves, and whole plants. Nitrogen fertilizer cultivation method, a total of 403 samples of ryegrass multiflora were killed at 105°C for 20 minutes, dried at 65°C, pulverized into powder in a micro pulverizer and passed through a 40-mesh sieve to obtain ryegrass multiflora sample powder;

[0054] b. The ryegrass multiflora sam...

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Abstract

The invention discloses a method for rapidly determining the content of crude protein in lolium multiflorum. According to the determining method, a prediction model, based on the near infrared spectrum technology, about the real content of the crude protein in the lolium multiflorumis provided, on the basis that the real content of a large number of samples is known, the near infrared original spectrum of a sample is acquired, the quantitative analysis prediction model, based on the near infrared spectrum technology and a chemical measuring method, about the content of the crude protein of the lolium multiflorum is established, then, the to-be-determined lolium multiflorum sample is pretreated, and to-be-determined lolium multiflorum sample powder is obtained; a near infrared original spectrum chart is acquired, the time of the spectrum acquisition process is short, and detection can be performed after the near infrared spectrum is acquired. The method has the characteristics of being simple to operate, rapid in detection, high in detection efficiency, high in detection accuracy and the like and is suitable for popularization and application in the field of pasture nutrition quality evaluation.

Description

technical field [0001] The invention relates to the field of forage nutritional quality evaluation, in particular to a method for measuring crude protein content of Lolium multiflora. Background technique [0002] Multifloral ryegrass (Lolium multiflorum Lam.) is an annual or perennial forage grass belonging to the Gramineae ryegrass family. It is suitable for growing in warm and humid regions. It is one of the excellent forage grasses with world cultivation significance. There are large areas of cultivation. In recent years, with the adjustment of the national agricultural industry structure, herbivorous animal husbandry has developed rapidly. Due to its high yield, strong growth in winter and spring, strong adaptability, and rich nutritional value, ryegrass multiflora has solved the problem of livestock feeding in winter and spring in southern my country. The problem of insufficient grass has played an extremely important role in the crop rotation of grain and grass in sou...

Claims

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359G01N2021/3595
Inventor 张新全杨忠富黄琳凯马啸潘玲汪霞黄婷许肖恒
Owner SICHUAN AGRI UNIV
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