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Strain of Laceyella sacchari capable of resisting acid, alkali, and alcohol and rapidly decomposing vinasse and applications thereof

A sugarcane Ranshiella, acid and alkali resistance technology, applied in the field of distiller's grains decomposing bacteria, can solve the problems of wasting manpower, material resources, financial resources and time, secondary pollution of distiller's grains and spoiled soil, low acid and alkali resistance of actinomycetes, etc. The environment and soil, the turbidity of the test tube is obvious, and the effect of preventing secondary pollution and corruption

Active Publication Date: 2017-07-04
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The actinomycetes disclosed in the above-mentioned patent documents have low acid and alkali resistance, poor alcohol resistance, and unsatisfactory temperature resistance, and are still not suitable for direct high-temperature composting of liquor distiller's grains
[0014] In summary, although there are many high-temperature decomposing bacteria on liquor distiller's grains disclosed, regardless of single-bacteria fermentation or mixed-bacteria fermentation, it is necessary to reduce the alcohol content and acidity of the distiller's grains. The process is complicated and wastes manpower, material resources, financial resources and time. , also can cause secondary pollution corruption and soil alkalization of distiller's grains at the same time, pollute environment, and, along with the carrying out of high-temperature decomposing process, the pH value of distiller's grains increases, continues to ferment, and alcohol content can continue to increase (3-6% )

Method used

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  • Strain of Laceyella sacchari capable of resisting acid, alkali, and alcohol and rapidly decomposing vinasse and applications thereof
  • Strain of Laceyella sacchari capable of resisting acid, alkali, and alcohol and rapidly decomposing vinasse and applications thereof
  • Strain of Laceyella sacchari capable of resisting acid, alkali, and alcohol and rapidly decomposing vinasse and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, purification, screening and identification of Laceyella sacchari (Laceyella sacchari) CP

[0037] 1) Separation and primary screening: Collect samples from high-temperature decomposed cow dung in Tangshan City, Hebei Province and bring them back to the laboratory. Take 10g of samples and add them to a sterilized Erlenmeyer flask with glass beads containing 100mL of cooled sterile water. Shake at 200r / min. The bed was oscillated and mixed for 30 minutes, then placed in a water bath at 80°C, and bathed in water for 10 minutes. -3 ~10 -9 Concentration, select 10 -3 , 10 -4 , 10 -5 , 10 -6 Use a pipette to draw 100 μL of each gradient suspension, spread it evenly on a nutrient agar plate (NA plate) with a spreader, use sterile water as a control, place the plate upside down in an incubator, and incubate at 50°C for 5 days , each treatment setting was repeated 3 groups horizontally, and according to the characteristics of microbial colony shape, siz...

Embodiment 2

[0068] Example 2 Test of tolerance to pH value of Laceyella sacchari (Laceyella sacchari) CP

[0069] Pick an appropriate amount of strains of Lancia saccharum CP slant and insert them into 100mL NA liquid medium, and cultivate them on a shaker at 50°C and 200r / min for 24 hours to obtain seed liquid. 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12 in NA liquid medium , 50°C, 200r / min shaker culture for 3 days, spread the NA plate, observe the growth; then put the seed solution into the NA liquid medium test tube with the same pH gradient at 50°C, 200r / min Incubate on a shaker for 3 days and observe the turbidity.

[0070] Test results: Ransiella sugarcane CP was cultured in NA liquid medium with a pH value of 3-12 at 50°C for 3 days, and the coated NA plate could grow well; it was cultured in NA liquid medium test tube at 50°C and 200r / min shaking table After 3 days, the growth was good, and the turbidity was obvious. Both tests pr...

Embodiment 3

[0071] Example 3 Alcohol tolerance test of Laceyella sacchari (Laceyella sacchari) CP:

[0072] Pick an appropriate amount of strains of Lancia saccharum CP slant and inoculate them into 100mL NA liquid medium, and culture them on a shaker at 50°C and 200r / min for 24 hours to obtain seed liquid. In 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 20% NA liquid medium, culture at 50°C, 200r / min for 3 days, spread NA plates, and observe the growth ; Then, the seed solution was added to the NA liquid medium test tube with the same alcohol concentration gradient at 1‰ inoculation amount, cultured on a shaker at 50°C and 200r / min for 3 days, and the turbidity was observed.

[0073] Test results: Lanxiella sugarcane CP was cultured in NA liquid medium with an alcohol content of 1-13% at 50°C for 3 days, and the coated NA plate could grow well; in the NA liquid medium test tube at 50°C, shaken at 200r / min After 3 days of bed culture, it grows well, and the turbidity is obvious. Both tests have pr...

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Abstract

The invention discloses a strain of Laceyella sacchari CP capable of resisting acid, alkali, and alcohol and rapidly decomposing vinasse and applications thereof. The preservation number of the strain is CGMCC No.13928. Laceyella sacchari can grow in a temperature range of 30 to 75 DEG C, and the optimal temperature for growth is 60 DEG C. Laceyella sacchari can generate multiple degrading enzymes such as protease, lipase, and the like, can well grow in a liquid / solid culture medium with an alcohol degree (V / V) of 1 to 13% and a pH value of 3 to 12, and is very suitable for a dynamic high temperature liquor vinasse decomposition technology, wherein in the technology, the pH rises, and the alcohol degree decreases and then rises. The treatments such as alcohol degree decreasing, acidity decreasing, and the like are not needed. The steps are simplified. Labor, materials, money, and time are saved. The temperature reaches 55 DEG C in 1d, 85 DEG C in 3d. The temperature of 83 to 85 DEG C is maintained for 3d. The fermentation period only lasts 15-16d. The output and quality of organic fertilizers are effectively improved. The secondary pollution and corruption of vinasse are prevented. The soil alkalization is avoided. The environment and soil are protected. A novel method is provided for producing high quality organic fertilizers by high temperature composting and decomposing of liquor vinasse.

Description

technical field [0001] The invention relates to distiller's lees decomposing bacteria, in particular to an acid-alkali-resistant and alcohol-resistant strain of Lancia saccharum rapidly decomposing distiller's grains and its application. Background technique [0002] Distiller's grains are the direct leftovers in the brewing process. At present, more than 50 million tons of distiller's grains need to be processed in my country. Therefore, people have been paying attention to the problem of disposing of distiller's grains after fermentation. Liquor distiller's grains are easily fermented and corrupted, and emit stench and easily pollute the environment. Because it contains a lot of crude fat, crude starch, crude protein and rich in nitrogen, phosphorus, potassium and polysaccharides, it is an excellent source of organic fertilizer. Therefore, the use of distiller's grains to make organic fertilizers can not only solve environmental problems, turn waste into treasure, but als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/01
CPCC05F5/008C05F17/00C12N1/205C12R2001/01C05F11/08Y02W30/40
Inventor 刘辉王灵敏李玲娣
Owner 陕西枫丹百丽生物科技有限公司
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