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Method for promoting chlorogenic acid accumulation in honeysuckle buds by utilization of fungi

A chlorogenic acid and fungus technology, applied in the fields of botanical equipment and methods, application, horticulture, etc., can solve the problems of unreported use, few leaf layer bacteria of thorn cotyledons, etc., and achieves wide source of raw materials, convenient operation, and improved quality effect

Active Publication Date: 2017-07-07
山东中平药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the fungus phyllophyllum, mainly to make it into food or medicinal raw materials, and other uses have not been reported.

Method used

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  • Method for promoting chlorogenic acid accumulation in honeysuckle buds by utilization of fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Adopt the following methods to cultivate honeysuckle and take flower buds:

[0031] 1. Preparation of honeysuckle cuttings

[0032] In early April, choose a cloudy day at around 9:00 am to collect cuttings, select two-year-old twigs or hard branches with a thickness of 4 mm, strong growth, no pests, no shrinkage, and no skin damage, and cut them to grow to about 30 cm. For cuttings, there are 6-8 nodes on each cutting, and 1 / 2 leaves are cut off from the leaves of each node. Tie the cut branches into 1 bundle every 50, and spray the branches with a sprayer. After the branches are all wet, put them into a foam heat insulation box, cover with a layer of wet towel to moisturize, cover the lid, and wait. cuttings.

[0033] , Preparation of fungal liquid

[0034] 2.1 Preparation of tea currant phyllodes fungus liquid

[0035] A. Preparation of Media

[0036] Preparation of slant medium: buy potatoes in the market, peel off the outer skin, cut into small pieces of 1 cm s...

Embodiment 2

[0057] According to the method of Example 1, honeysuckle was cultivated, and flower buds were collected, except that the Trichoderma strain used was Trichoderma viride JUWE1002 with the preservation number of CGMCC NO.7035.

Embodiment 3

[0059] According to the method of embodiment 1, honeysuckle is cultivated, and flower buds are collected, and the difference is that the Trichoderma strain used is Trichoderma harziamum (Trichoderma harziamum) YS-JZ0386 (purchased from Shanghai Yansheng Industrial Co., Ltd.) preserved in the American Type Culture Collection. company).

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Abstract

The invention discloses a method for promoting chlorogenic acid accumulation in honeysuckle buds by utilization of fungi. According to the method, honeysuckle is cultivated by using a cutting seedling method, and fungi inoculation is carried out on honeysuckle branches during the cutting process to increase the content of chlorogenic acid in the honeysuckle buds; and the fungi are Phylloporia ribis and Trichoderma. Honeysuckle plants are processed by the fungi, the fungi grow with growth of the honeysuckle, the fungi grow at the basal stem of the honeysuckle, the growth of the fungi can promote accumulation of secondary metabolites in the honeysuckle buds, and quality of honeysuckle flowers is increased. The method is wide in raw material source, convenient to operate, and easy to carry out, and has great signification for increasing the honeysuckle flower quality in production.

Description

technical field [0001] The invention relates to a method for promoting the accumulation of secondary metabolites in honeysuckle flower buds, in particular to a method for promoting the accumulation of chlorogenic acid in honeysuckle flower buds by using fungi. Background technique [0002] With the surge of the trend of "returning to nature", traditional natural herbal medicines are becoming more and more popular. Honeysuckle comes from Lonicera Lonicerae Lonicera japonica Thunb.'s dried flower buds or early bloomed flowers are cold in nature and sweet in taste. It has the effect of clearing heat and detoxifying, cooling and dispelling wind-heat. It is a commonly used medicine in traditional Chinese medicine. According to statistics, honeysuckle is used in about 1 / 3 of traditional Chinese medicine prescriptions, and It is widely used in Chinese patent medicines such as Shuanghuanglian, Yinqiao Jiedu Pills, and Yinhuang Oral Liquid. It has played a huge role in the preventi...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G9/10
CPCA01G9/0299A01G22/00
Inventor 周洁方磊张华
Owner 山东中平药业有限公司