Hormone receptor-positive breast cancer recurrence monitoring gene mutation library construction method
A technology of hormone receptors and construction methods, applied in biochemical equipment and methods, microbial measurement/inspection, DNA preparation, etc., can solve problems such as interference, treatment decision-making troubles, and detection method distortion
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[0144] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0145] Construction method of gene mutation library for hormone receptor positive breast cancer recurrence monitoring, covering human genes KRAS, JAK3, AKT1, CREBBP, PTEN, RB1, TP53, CTNNB1, TSC2, TSC1, ERBB2, PIK3CA, SF3B1, JAK2, CDH1, SMAD4, A total of 1357 somatic mutations in GATA3, MAP2K4, MAP3K1 and ESR1.
[0146] Specifically include the following steps:
[0147](1) Design basic amplification primers for target genes KRAS, JAK3, AKT1, CREBBP, PTEN, RB1, TP53, CTNNB1, TSC2, TSC1, ERBB2, PIK3CA, SF3B1, JAK2, CDH1, SMAD4, GATA3, MAP2K4, MAP3K1 and ESR1 The 5' end of the forward primer and the reverse primer of the basic amplification primer set is provided with an additional 2-5 Ts, and the first T of the 2-5 Ts near the 3' end has a PNA modification, At the same time, the Tm value difference of the basic amplification primer se...
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