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Device suitable for sogatella furcifera feeding method RNA interference experiment and using method thereof

A technology of RNA interference and white-backed planthopper, which is applied in the field of RNA interference experiment of white-backed planthopper feeding method, can solve the problems that the gene function control technology of white-backed planthopper has not been reported, and achieves the convenience of feeding feed, The effect of simple operation and reasonable structure

Pending Publication Date: 2017-07-14
ZUNYI MEDICAL UNIVERSITY
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Problems solved by technology

[0003] Since its discovery, RNAi technology has been widely used in the study of insect gene functions, such as Drosophila RNAi, termite RNAi and Italian bee RNAi, but the gene function control technology for white-backed planthopper has not been reported

Method used

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  • Device suitable for sogatella furcifera feeding method RNA interference experiment and using method thereof

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Embodiment Construction

[0015] The present invention will be described in further detail below by means of specific embodiments:

[0016] The specific implementation of the present invention is illustrated by the following examples, but the protection scope of the present invention is not limited thereto.

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Abstract

The invention discloses a device suitable for a sogatella furcifera feeding method RNA interference experiment. The device comprises a hollow and transparent glass tube, opening sealing layers and a light-proof sleeve, wherein the light-proof sleeve is arranged in the middle of the outer surface of the glass tube in a sleeving mode, the opening sealing layers are arranged at the two ends of the glass tube, fodder layers are arranged on the opening sealing layers, dsRNA and artificial fodder are arranged in the fodder layers, and the dsRNA is double-stranded RNA of a specific gene of sogatella furcifera. A characteristic that insects utilize optical driving is utilized, the sogatella furcifera moves to the two ends of the glass tube and reaches the opening sealing layers, the artificial fodder and the dsRNA are fixed in the opening sealing layers through two layers of films, the sogatella furcifera can pierce thinned Parafilm opening sealing films through mouthparts, and convenience is brought to the sogatella furcifera to take fodder and corresponding dsRNA. In addition, research specialist staff can conveniently observe sogatella furcifera phenotypic change such as death rate, malformation and the like by arranging the transparent glass tube after some gene expression is silenced by a feeding method RNA interference technology, and a sogatella furcifera gene function researching technology can be further obtained.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to the technical field of auxiliary tools for gene function research of the important rice pest white-backed planthopper, and more particularly to a device suitable for RNA interference experiment of feeding method of white-backed planthopper and its use method. Background technique [0002] Rice is an important food crop in my country, and its output ranks first among various food crops. The white-backed planthopper is one of the important migratory pests of rice in my country. It sucks rice through the piercing-sucking mouthparts, causing slow growth of rice, delaying tillering, and increasing shriveled grains. production poses serious hazards. RNA interference, also known as RNAi, is a gene silencing phenomenon induced by double-stranded RNA (dsRNA). The double-stranded RNA is first cut into 19-25bp siRNA by Dicer enzyme, and then the siRNA is combined with the RNA-induced...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K20/153
CPCA01K67/0339A01K2207/05A01K2227/706
Inventor 陈静张道伟
Owner ZUNYI MEDICAL UNIVERSITY
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