A method of reducing lipid blood sample detection interferences

Abstract

The invention relates to a method of reducing lipid blood sample detection interferences. The method includes a step of preparing a lipase buffer solution, namely a step of weighing 1-2.5 g of lipase and 1-2 g of auxiliary lipase, measuring 4-10 mL of a dipotassium phosphate-monopotassium phosphate buffer liquid, and fully mixing and dissolving the lipase, the auxiliary lipase and the buffer liquid in a glass container, with the lipase activity of the prepared solution being higher than 500 [mu]/L; a step of adding 5-10% of the prepared lipase buffer solution into a lipid blood sample and performing incubation for 10-20 min; and a step of performing centrifugal treatment by adopting a centrifuge, with the rotating speed of the centrifuge being controlled to be 1800-3000 r/min. The method does not need a high-requirement expensive supercentrifuge, a detecting process is simple and a detection result is small in error.

Description

technical field [0001] The invention relates to a method for reducing interference in the detection of lipid blood samples. Background technique [0002] The current biochemical analyzers use spectrophotometry for the detection of blood samples, and the lipid blood samples in blood samples have obvious scattering effects on light of various wavelengths, so it usually has a great impact on the results of biochemical analyzers, resulting in The biochemical analyzer test results are inaccurate. However, in the current detection of lipid blood samples, ultracentrifugation or dilution methods are usually used to process the lipid blood samples before detection, so as to reduce the influence of lipid blood on the test results. However, these two detection methods have disadvantages: Among them, the ultracentrifugation method is to process the blood lipid and serum of the lipid blood sample into layers through an ultracentrifuge, and then perform separate detection. Using this met...

AI Technical Summary

Problems solved by technology

However, these two detection methods have disadvantages: Among them, the ultracentrifugation method is to process the blood lipid and serum of the lipid blood sample into layers through an ultracentrifuge, and then perform separate detection. Using this method, the ultracentrifuge is expensive, and the general experiment The room cannot be equipped, and other substances in the blood are also stratified after ultracentrifugation, that is to say, there are not only two layers of blood lipid and serum, which will cause trouble in detection; the dilution method is to ensure the light transmittance of spectrophotometry , dilute the lipid blood sample to a relatively dilute concentration, and the test results of the relatively dilute concentration of the lipid blood sample directly have a large error