Rice OsMts1 gene and coding protein and application thereof

A gene and protein technology, applied in the field of rice OsMts1 gene and its encoded protein and its application, can solve the problems of premature aging of rice

Active Publication Date: 2017-07-21
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the rice database annotation website (www.rice.plantbiology.msu.edu), there are a large number of methyltransferases in rice, but there is no report on the regulation of rice premature senescence

Method used

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  • Rice OsMts1 gene and coding protein and application thereof
  • Rice OsMts1 gene and coding protein and application thereof
  • Rice OsMts1 gene and coding protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Rice material:

[0047] Rice material (Oryza stiva L.) mutant pls1, the original material is the indica rice restorer variety "Zhonghui 8015".

[0048] Rice premature senescence mutant pls1 was obtained from Zhonghui 8015 60 Co-γ radiation induced induction (such as figure 1 shown). The rice progeria mutant material is currently preserved in the China Rice Research Institute.

[0049] 2. Determination of photosynthetic rate:

[0050] Determination of photosynthetic rate The two-month-old progeria mutant pls1 and wild type planted in the field were used to measure the photosynthesis of rice flag leaves with a photosynthetic instrument (Li-6400) at two o'clock in the afternoon and under the light intensity of the day, and each material was 8 repetitions.

[0051] 3. Determination of hydrogen peroxide content:

[0052] Hydrogen peroxide can form a complex under the action of molybdic acid, and the amount of H can be calculated at 405nm. 2 o 2 amount.

[0053] T...

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Abstract

The invention discloses a rice OsMts1 gene and a coding protein and application thereof. The rice OsMts1 gene is obtained by utilizing a map-based cloning technology and cloning a pls1 mutant of 'Zhong-Hui 8015' of a 60 Co-gamma radiation induced mutation indica type rice variety. It is proved in the pls1 mutant through a transgene function complementation experiment that the gene controls rice premature senility. In addition, it is found through gene function analysis that the gene affects chloroplast development by regulating and controlling active oxygen and thus causes leaf premature senility. The gene and its coding protein can be applied to breeding of premature senility resistant plant varieties.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a rice OsMts1 gene, its encoded protein and its application. Background technique [0002] Hybrid rice usually produces late senescence in production, which seriously affects the photosynthetic efficiency of hybrid rice, and ultimately affects yield and quality, posing a certain threat to my country's food security. Therefore, revealing the physiological and biochemical mechanism of rice premature senescence can provide a theoretical basis for breeding hybrid rice resistant to premature senescence. There are many factors that affect premature aging. Generally speaking, the redox balance in plants is out of balance (simply speaking, the active oxygen in cells accumulates in large quantities), which easily leads to leaf aging. Therefore, the antioxidant mechanism of plants is particularly important. Substances such as SOD, POD and melatonin play an important role in scavenging ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00
CPCC12N9/1007C12N15/8205C12N15/825C12N15/8261C12N15/8266
Inventor 洪永波张迎信曹立勇程式华占小登沈希宏陈代波于萍吴玮勋
Owner CHINA NAT RICE RES INST
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