Application of SFN (sulforaphane) to preparation of medicine for treating leukemia

A technology of sulforaphane and leukemia, which is applied in the field of application of sulforaphane in the treatment of leukemia drugs, and can solve the problems of less reports on the role and mechanism of hematopoietic tumor cells

Inactive Publication Date: 2017-08-04
XINXIANG MEDICAL UNIV +1
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  • Abstract
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Problems solved by technology

However, there are few reports on the effect an

Method used

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  • Application of SFN (sulforaphane) to preparation of medicine for treating leukemia
  • Application of SFN (sulforaphane) to preparation of medicine for treating leukemia
  • Application of SFN (sulforaphane) to preparation of medicine for treating leukemia

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Embodiment

[0017] 1. Experimental materials

[0018] Sulforaphane (batch number 28911701) was purchased from LKT Laboratories, USA, with a purity of 98%. 1640 culture medium and fetal bovine serum are products of Gibco Company in the United States. CCK-8 kit was purchased from Dojindo Company in Japan, Annexin-V / PI apoptosis detection reagent (BD Company, USA, batch number 5121706), RNA extraction kit, reverse transcription kit, PCR reagent 2×Taq PCRMaster Mix, DNA Marker All were purchased from Shanghai Tiangen, agarose (Biowest, Spain), primers were synthesized by Shanghai Bioengineering Company, rabbit anti-human Bax mAb, rabbit anti-human Bcl-2 mAb, rabbit anti-human Caspase-3 mAb were produced by American Abcam Company, anti-human Human GAPDH pAb and HRP-goat anti-rabbit pAb were provided by Century Kangwei Company. The flow cytometer (FACSCaibur type) is produced by BD Company in the United States, the MK3 microplate reader and ND2000 ultra-micro spectrophotometer are produced by...

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Abstract

The invention discloses application of SFN (sulforaphane) to preparation of medicine for treating leukemia, and belongs to the technical field of novel purpose of the SFN. The application is characterized in that according to the application of the SFN to the preparation of the medicine for treating the leukemia, the SFN promotes the leukemia apoptosis through raising Bax genes and Caspase-3 genes and reducing Bcl-2 genes for further inhibiting the multiplication of leukemia cells. The influence of the SFN on the acute leukemia cell KG1a and K562 apoptosis is systematically discussed for the first time; a result proves that the SFN can promote the acute leukemia apoptosis through regulating and controlling the Bax genes, the Bcl-2 genes and the Caspase-3 genes; the result belongs to the theoretical breakthrough and has the potential clinic application significance; a fire-new thought and potential treatment path is provided for leukemia treatment.

Description

technical field [0001] The invention relates to the technical field of new uses of sulforaphane, in particular to the application of sulforaphane in medicines for treating leukemia. Background technique [0002] Sulforaphane (SFN) is an isothiocyanate substance present in broccoli and other cruciferous vegetables, which has anti-proliferative ability. It has inhibitory effect on the growth of malignant tumor cells and is a new type of anti-cancer ingredient. However, the effect and mechanism of SFN on hematopoietic tumor cells are less reported. [0003] Uncontrolled cell cycle is one of the important causes of malignant proliferative diseases. Acute leukemia is a malignant clonal disease of hematopoietic stem cells. Leukemia cells proliferate massively in bone marrow and other hematopoietic tissues due to uncontrolled proliferation, impaired differentiation, and blocked apoptosis, and infiltrate other tissues and organs, while normal hematopoiesis is inhibited. The Bcl-2...

Claims

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Application Information

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IPC IPC(8): A61K31/145A61P35/02
CPCA61K31/145
Inventor 王凡平王明永王闪闪康丽霞乔瑞娟李克君王艳于海川牛志国
Owner XINXIANG MEDICAL UNIV
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