A method for identifying low temperature resistance characteristics of rape seed germination and seedling growth

Abstract

The invention belongs to the technical field of breeding and cultivation of rape seeds, and particularly relates to a method for identifying low temperature resistance during germination and seeding formation of rape seeds. The method comprises the following steps: performing germination experiment on screened and disinfected rape seeds in an illumination incubator of 10-5 DEG C; continuously culturing for 21 days; measuring eight indexes of a germination rate, a seedling rate, average germination time, a germination index, root length, root fresh weight, total fresh weight and root dry weight, and making related membership function analysis; and performing scientific classifying for evaluating low temperature resistance of the rape speeds according to low-temperature-resistant grading standards during germination and seeding formation of the rape speeds. The method is simple and easy to implement, saves expenditure, is accurate in result, and can be used for accurately identifying and screening low-temperature-resistant rape varieties.

Description

technical field [0001] The invention belongs to the technical field of low-temperature resistance of rape seeds, and in particular relates to a method for identifying the low-temperature resistance characteristics of rapeseed germination and seedlings. Background technique [0002] Due to the transfer of rural labor force in China, the need for cost-saving and simplified cultivation, and the development of mechanized planting, the sowing date of winter rapeseed in the Yangtze River Basin in China has been postponed in recent years, which directly leads to the risk of low temperature when rapeseeds germinate in this area. As we all know, in the cultivation practice of crops, "germination of seedlings" is the first stage of the whole growth period of crops, and it is also one of the most important stages. Sowing all seedlings and seedlings equal to and strong will directly affect the formation of rapeseed yield, and even affect the rape planting area in the entire Yangtze Rive...

AI Technical Summary

Problems solved by technology

[0003] The previous studies on the low-temperature germination of rape seeds were relatively single, not systematic and in-depth, and there were very few studies on a large number of varieties

For example, Xian Mengzhu et al. (Xian Mengzhu, Evaluation of Low Temperature Resistance of Rapeseed Germination and Seedling Stage, Journal of Crops, 2015(5): 116‐122) carried out the evaluation of low temperature resistance of rapeseed The method of germination and seedling ability, but at present, the workload of this method is relatively large (temperature settings are 25 / 20°C, 10 / 5°C), and the investigation and calculation methods are cumbersome (investigation and calculation of 18 indicators, some of which are not original indicators, and the ratio of low temperature to control was used for principal component analysis), the coverage of survey indicators was not strong (the coverage of the six indicators of principal component analysis was not strong), and the grading standards were not very scientific (the grading standard of corn drought resistance was used for reference, not too scientific)

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