Eugenol combination liquid as well as preparation method and application thereof

A technology for eugenol and a combined solution is applied in the field of eugenol combined solution and preparation, which can solve problems such as adverse effects on the natural ecological environment, and achieve the effects of ensuring human health, avoiding bacterial contamination, and reducing usage rates.

Inactive Publication Date: 2017-08-08
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Long-term use of chemical preservatives may lead to potential safety issues such as carcinogenici...

Method used

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  • Eugenol combination liquid as well as preparation method and application thereof
  • Eugenol combination liquid as well as preparation method and application thereof
  • Eugenol combination liquid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Preparation of eugenol combination liquid A

[0015] Add 94.5 parts of eugenol to 50 parts of autoclaved Tween 80 solution, dissolve the eugenol by ultrasonic vibration, then pass the solution through a 0.22 μL disposable filter head to filter and sterilize, and finally add 99855.5 parts of water to dilute.

Embodiment 2

[0016] Embodiment 2: Preparation of eugenol combination liquid B

[0017] Add 85.05 parts of eugenol to 45 parts of autoclaved Tween 80 solution, dissolve the eugenol by ultrasonic vibration, then pass the solution through a 0.22 μL disposable filter head to filter and sterilize, and finally add 99868.95 parts of water to dilute.

Embodiment 3

[0018] Embodiment 3: Preparation of eugenol combination liquid C

[0019] Add 75.6 parts of eugenol to 40 parts of autoclaved Tween 80 solution, dissolve the eugenol by ultrasonic vibration, then pass the solution through a 0.22 μL disposable filter head to filter and sterilize, and finally add 99884.4 parts of water to dilute.

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Abstract

The invention provides a eugenol combination liquid as well as a preparation method and an application of the eugenol combination liquid. The combination liquid comprises the following raw materials by volume: 99.8555%-99.9711% of water, 0.01%-0.05% of Tween 80 solution, and 0.0189%-0.0945% of eugenol; and the invention provides the method for preparing the eugenol combination liquid. The eugenol combination liquid inhibits the growth of staphylococcus aureus, and can even be used as a bactericide when the concentration of the eugenol is higher. The eugenol combination liquid affects the molecular structures of an SDH enzyme and an MDH enzyme of staphylococcus aureus, and the staphylococcus aureus is changed, so that the conformation is changed, and the activity of the enzyme is reduced. After the eugenol combination liquid acts on the staphylococcus aureus, the nucleic acid substances related to the synthesis of protein are leaked because of the change of cell permeability, the synthesis of the protein is further inhibited, and finally the cell death is caused.

Description

technical field [0001] The invention relates to a eugenol combination liquid, a preparation method and application. Background technique [0002] As the topic of food safety continues to receive attention, people have higher requirements for food quality, not only for nutrition, but also for green and healthy food. In order to facilitate food transportation and storage, people often add preservatives to food, such as benzoic acid, sorbic acid and its salts, parabens, propionates, sulfur dioxide, sulfurous acid and its salts, nitrates and nitrous acids Salts, etc., have been widely used. The long-term use of chemical preservatives may cause potential safety problems such as carcinogenicity, mutagenicity, and teratogenicity, and will also adversely affect the natural ecological environment. Therefore, searching for broad-spectrum, high-efficiency, low-toxicity and safe food preservatives has become one of the hotspots in the field of food research. [0003] Staphylococcus a...

Claims

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Application Information

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IPC IPC(8): A23L3/349
CPCA23L3/349A23V2002/00A23V2200/10A23V2250/2132
Inventor 曾荣李海贤马艳玲
Owner FOSHAN UNIVERSITY
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