Interconnections of multiple perfused engineered tissue constructs and microbioreactors, multi-microformulators and applications of the same
An interconnected bus, biological technology, applied in the field of microfluidic systems, can solve problems such as cost constraints
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Embodiment 1
[0161] Systems and methods for aseptic fluidic interconnection of multiple perfused engineered tissue constructs and microreactors
[0162] Some aspects of the present invention relate to systems and methods for aseptic fluid interconnection of multiple perfused engineered tissue constructs and microreactors, particularly the interconnection of integrated organ microfluidics (IOM) modules to create a human body-on-a-chip. In some embodiments, the present invention addresses the issues of sterilization, fluid containment, bubble generation, and excess volume associated with standard techniques for connecting organs using simple microfluidic channels or tubing. The novelty of the present invention is that the interconnection is arranged as a parallel circuit with cleaning line, arterial line, venous line and sewage line. The present invention has potential value for long-term culture of tissue engineered organ construction in any system or process, especially for processes that ...
Embodiment 2
[0219] Push-pull injectable microformulation module with multiple open reservoirs
[0220] The miniformulation module 100 shown above in Figures 1 and 17 is described in PCT Publication Nos. WO 2012 / 048261, WO 2013 / 086505, and WO 2014 / 081840, and is used to prepare and deliver custom formulations of cell culture media, including pharmaceuticals, Toxins and growth factors whose output is delivered to a single bioreactor, organ-on-a-chip or other fluidic reservoir, or a single set of such interconnected devices. The following describes how a computer-controlled microfluidic microformulation module, with additional computer-controlled valves and a fluid distribution and collection network, can be used to deliver solutions or mixtures of solutions independently or into multiple fluid reservoirs, such as for cell culture Well plates, multiple tissue or cell culture bioreactors or in different organs in a connected, in vitro organ-on-a-chip microphysiological system, and fluid is re...
Embodiment 3
[0308] Device for fluidic control between engineered tissue constructs, microreactors and well plates
[0309] This example discusses how the previously described invention can be modified and optimized for the control of fluids, as is specifically required for long-term cell culture in tissue chips or organ chips, which can be considered as two-dimensional or three-dimensional microreactions for culturing adherent or non-adherent cells with and without a supporting matrix.
[0310] When organ chips are connected together, there is a need for the missing endocrine organ system microscopic formulation design module, which can provide endocrine control for the above-mentioned integrated organ chip. Figure 36 A schematic of one such system comprising the neurovascular unit / blood-brain barrier, liver, kidney and skeletal muscle is shown. Control and sensing functions can be provided by different modules such as Figure 17 mentioned. For perspective, we are developing an in vit...
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