Dual-channel structure optical digital phase contrast microscopy imaging system and realization method thereof

Abstract

The present invention discloses a dual-channel structure optical digital phase contrast microscopy imaging system and a realization method thereof, wherein the system comprises a light source, a beam expansion collimating unit, a light splitter, a lens group and two same cameras. The beam expansion collimating unit is used to adjust the divergent light emitted out by the light source into the parallel light and irradiate to a to-be-detected object, the light splitter, the lens group and the two cameras form two sets of symmetrical 4f imaging systems and obtain two synchronization images to calculate a real-time bright field micrograph, a differential phase contrast graph and a quantitative phase diagram image. Based on utilizing the common and cheap devices in the market specifically, and by processing simply, a same phase contrast imaging effect can be realized, and the problems that the devices are expensive, and a spatial light modulator is sensitive to the polarization of the light, are solved.

Description

technical field [0001] The invention relates to a dual-channel structured light digital phase-contrast microscopic imaging system and a realization method thereof, belonging to the technical field of microscopic imaging. Background technique [0002] Phase contrast imaging technology is a microscopic imaging technology that can convert the small optical path difference at different positions of the object into intensity difference. Since Zernik invented the phase contrast microscope in 1935 (reference paper: F Zernik. Phase contrast, a new method for the microscopic observation of transparent objects. Physica, Vol.9, No.7), phase contrast microscopy has been widely used At the same time of application, new phase contrast imaging technology is constantly being proposed. The Laura Waller research group of the Berkeley National Laboratory at the University of California, Berkeley proposed a structured light illumination digital phase contrast technology based on array light so...

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Problems solved by technology

However, according to the introduction in the literature, this method uses a spatial light modulator to modulate the light in the spectral plane of the imaging optical path, which is expensive, and the refresh rate of the spatial light modulator limits the frame rate of image acquisition, so it cannot be applied to high-speed imaging. Phase contrast imaging of the changing process, in addition, there is a problem that the polarization state of the light affects the modulation effect of the spatial light modulator on the light

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