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Method for preparing heavy metal removed pea protein from pea starch wastewater

A technology of pea protein and pea starch, which is applied in the field of pea protein with high protein content, and can solve the problems of complex removal of heavy metals

Active Publication Date: 2017-08-18
WUXI JINNONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention combines the fermentation process with the preparation process of pea protein, and utilizes the fermentation process and fermentation products to obtain refined pea protein for removing heavy metals, which overcomes the defects of the current complicated process for removing heavy metals from pea protein

Method used

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  • Method for preparing heavy metal removed pea protein from pea starch wastewater
  • Method for preparing heavy metal removed pea protein from pea starch wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The pea starch wastewater solution is subjected to high-pressure instantaneous sterilization, and is sent into a mechanically stirred and ventilated fermenter, and 5% seeds of Gluconoacetobacter xylinum are inserted, and the temperature is fermented at 25°C for 96h. After the fermentation is over, the pH of the fermented liquid At 4.8, obtain the pea starch wastewater fermented liquid containing microaggregate bacterial cellulose and organic acid;

[0023] (2) The above-mentioned fermented liquid is separated by a centrifuge with a centrifugal force of 3000×g to obtain a supernatant and a precipitate. The supernatant is a fermented organic acid solution chelated with heavy metals, and the precipitate is organic acid chelated and removed once. Heavy metal pea protein, Gluconacetobacter xylinum cells and bacterial cellulose;

[0024] (3) Send the precipitate prepared in step (2) into the slurry tank, add 3 times of water to adjust the slurry, add lye to adjust the pH ...

Embodiment 2

[0031] (1) The pea starch wastewater solution is sterilized by high pressure instantaneously, sent into a mechanically stirred and ventilated fermenter, inserted with 20% gluconoacetobacter xylinum seeds, and fermented at 37° C. for 5 hours. After the fermentation, the pH of the fermented liquid At 4.0 to 4.8, the pea starch wastewater fermentation liquid containing microagglomerated bacterial cellulose and organic acids is obtained;

[0032] (2) The above-mentioned fermented liquid is separated by a centrifuge with a centrifugal force of 2500×g to obtain a supernatant and a precipitate. The supernatant is a fermented organic acid solution chelated with heavy metals, and the precipitate is organic acid chelated and removed once. Heavy metal pea protein, Gluconacetobacter xylinum cells and bacterial cellulose;

[0033] (3) Put the precipitate prepared in step (2) into the slurry tank, add 1 times of water to adjust the slurry, add lye to adjust the pH to 8.5, at 40°C, stir at a...

Embodiment 3

[0040] (1) The pea starch wastewater solution is sterilized by high pressure instantaneously, sent into a mechanically stirred and ventilated fermenter, inserted with 10% gluconoacetobacter xylinum seeds, and fermented at 20° C. for 48 hours. After the fermentation, the pH of the fermented liquid In 4.4, obtain the pea starch wastewater fermented liquid that contains microaggregate bacterial cellulose and organic acid;

[0041](2) The above-mentioned fermented liquid is separated by a centrifuge with a centrifugal force of 2800×g to obtain a supernatant and a precipitate. The supernatant is a fermented organic acid solution chelated with heavy metals, and the precipitate is organic acid chelated and removed once. Heavy metal pea protein, Gluconacetobacter xylinum cells and bacterial cellulose;

[0042] (3) Send the precipitate prepared in step (2) into the slurry tank, add 2 times of water to adjust the slurry, add lye to adjust the pH to 10.0, and dissolve the pea protein at ...

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Abstract

The invention discloses a method for preparing heavy metal removed pea protein from pea starch wastewater by virtue of a fermentation method. The pea starch wastewater is fermented to generate organic acid to chelate primary heavy metals by virtue of gluconacetobacter xylinus, secondary heavy metals in the pea protein are adsorbed by virtue of thalli of the gluconacetobacter xylinus and bacterial cellulose generated by the gluconacetobacter xylinus, and tertiary heavy metals in the pea protein are finally eluted by acid precipitates in a pea protein refining process. According to the method, the pea protein in which heavy metals reach the standard and which are high in protein content is obtained by combining refining of the pea protein from the pea starch wastewater with the fermentation method and three-step removal of the heavy metals in the pea proteins.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for refining pea protein from pea starch wastewater by using a fermentation method combined with three-step removal of heavy metals in the pea protein to obtain pea protein with high protein content that meets the heavy metal standard. Background technique [0002] Pea protein is a high-quality protein with a relatively balanced proportion of amino acids and a digestibility rate of more than 98%. Among the eight essential amino acids required by the human body, except for the slightly lower content of methionine, the rest have reached the recommended model value of FAO / WHO. Pea protein is a non-allergic protein and does not contain anti-nutritional factors. Therefore, we can see that pea protein itself has a high added value. Can be widely used in health care products, meat products, beverages, baked goods, dairy products, etc. Legumes do not contain cholesterol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/16
CPCA23J1/16A23V2002/00A23V2300/02A23V2250/5108
Inventor 于秋生徐珍珍陈林冯伟陈天祥李珍妮朱熹
Owner WUXI JINNONG BIOTECH CO LTD
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