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The medical use of prohematoxylin a

A technology of hematoxylin and Brazilian hematoxylin, which can be applied in the fields of medicine and pharmacy, and can solve the problems such as the effect report that has not yet been seen

Active Publication Date: 2020-01-14
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the effect of prohematoxylin A on ischemic stroke

Method used

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  • The medical use of prohematoxylin a
  • The medical use of prohematoxylin a
  • The medical use of prohematoxylin a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Isolation and identification of protohematoxylin A

[0042] Hematoxylin dried heartwood (21kg) was cut into small thin strips, followed by reflux extraction with 8 times, 6 times and 6 times of 95% ethanol for 3 times, 1h each time; After volatilizing the solvent to no alcohol smell, it was suspended in water and extracted with petroleum ether, ethyl acetate and n-butanol in turn. After recovering the solvent, 60 g of petroleum ether extract, 1400 g of ethyl acetate extract, and n-butanol extraction were obtained. 360g.

[0043] Take 800 g of ethyl acetate extract, and perform column chromatography on silica gel (100-200 mesh), chloroform-methanol=30:1, 20:1, 10:1, 5:1, 1:1 gradient elution, TLC comparison and combination Afterwards, 14 fractions of Fr.A-Fr.N were obtained. Fraction Fr.E was subjected to silica gel (200-300 mesh) column chromatography, petroleum ether-ethyl acetate (3:1, 2:1, 1:1) to obtain fractions Fr.E-B, Fr.E-C, Fr.E-E , Fr.E-F. Frac...

Embodiment 2

[0058] Example 2 Protective effect of protohematoxylin A on cerebral ischemia-reperfusion injury in rats

[0059] 2.1 Test animals: SD rats, 8 weeks, male, Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.

[0060] 2.2 Test drugs:

[0061] Pro-hematoxylin A (PTA), dissolved in 0.5% CMCNa solution to prepare a 5 mg / mL drug solution.

[0062] Protohematoxylin B (PTA), dissolved in 0.5% CMCNa solution to prepare a 5 mg / mL drug solution.

[0063] 2.3 Animal grouping and handling:

[0064] Forty SD rats, 8 rats in each group, were randomly divided into 5 groups after 1 week of adaptive feeding: sham group (sham operation group, only the outer epidermis was incised but no arterial ligation), model group (model group), PTA low dose group (20mg / kg), PTA high dose group (50mg / kg) and PTB administration group (50mg / kg). Except for the normal control group, the animals in each group underwent the following operations:

[0065] The right common carotid artery (CCA), inte...

Embodiment 3

[0077] Example 3 Improvement of neurobehavioral effects of protohematoxylin A on cerebral ischemia-reperfusion in rats

[0078] 3.1 Test animals: SD rats, 8 weeks, male, Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.

[0079] 3.2 Test drugs:

[0080] Pro-hematoxylin A (PTA), dissolved in 0.5% CMCNa solution to prepare a 5 mg / mL drug solution.

[0081] Protohematoxylin B (PTA), dissolved in 0.5% CMCNa solution to prepare a 5 mg / mL drug solution.

[0082] 3.3 Animal grouping and handling:

[0083]Forty SD rats, 8 rats in each group, were randomly divided into 5 groups after 1 week of adaptive feeding: sham group (sham operation group, only incision of the epidermis but no arterial ligation), model group, PTA low-dose group ( 20mg / kg), PTA high dose group (50mg / kg) and PTB administration group (50mg / kg). Animals in each group underwent the following operations:

[0084] The right common carotid artery (CCA), internal carotid artery (ICA) and external carotid ...

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Abstract

The invention relates tomedical application of protosappanin A, in particular to application of protosappanin A to the preparation of drugs for treating ischemic cerebral apoplexy. The invention further provides application of protosappanin A to the preparation of drugs for preventing and / or treating acute and chronic complications caused by cerebral ischemia.

Description

technical field [0001] The invention belongs to the field of medicine and pharmacy, and specifically relates to a new medical application of prohematoxylin A. Background technique [0002] Ischemic stroke is a serious cerebrovascular disease that causes acute neuronal necrosis due to decreased blood perfusion in certain areas of the central nervous system, which in turn causes irreversible damage to brain tissue and the entire neurological function. At present, ischemic stroke has the characteristics of high morbidity, high mortality, high disability rate and many complications in the elderly. With the improvement of people's living standards and the aggravation of population aging, ischemic stroke has become a major fatal disease, causing serious burdens to patients and society. Most of the current clinical drugs for ischemic stroke can only provide partial protection, the curative effect is not significant, and it is accompanied by different degrees of side effects. Ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P25/28A61P9/10A61P25/00
CPCA61K31/365
Inventor 屠鹏飞曾克武赵明波姜勇
Owner PEKING UNIV