Real-time fluorescent quantitative PCR (polymerase chain reaction) gene amplification detector

A technology of real-time fluorescence quantification and gene amplification, which is applied in the measurement/inspection of microorganisms, biochemical instruments, bioreactors/fermenters for specific purposes, etc. Increase and prevent the effect of electrostatic breakdown

Pending Publication Date: 2017-08-18
滨江华康(北京)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the hot cover device makes the instrument bulkier and more complex in structure

Method used

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  • Real-time fluorescent quantitative PCR (polymerase chain reaction) gene amplification detector
  • Real-time fluorescent quantitative PCR (polymerase chain reaction) gene amplification detector
  • Real-time fluorescent quantitative PCR (polymerase chain reaction) gene amplification detector

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0027] Such as Figure 1~3As shown, the present invention includes a bottom casing 100 and a gene amplification module 200 arranged in the bottom casing 100. The gene amplification module 200 includes an insulating sheet metal 1 in the shape of "pin" and is arranged on the top surface of the insulating sheet metal 1. The heating plate 2 is provided with a shelving cylinder 3 with a closed top and an open bottom. The closed end of the shelving cylinder 3 is provided with a concave and inverted tapered reagent hole 4. The heat emitted by the heating plate 2 can be transferred to the reagent. Well 4, so DNA fragments can complete the processes of denaturation, annealing and extension in reagent well 4 to realize gene amplification. A fluorescence detection module 300 is detachably arranged above the gene amplification module 200. The fluorescence detect...

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Abstract

The invention relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) gene amplification detector, characterized by comprising a bottom shell and a gene amplification module disposed in the bottom shell; the gene amplification module comprises an insulating metal plate in 'A' shape and a heating plate arranged at the top of the insulating metal plate, a holding barrel with the top sealed and the bottom opened is arranged on the heating plate, a recessed inverted-cone-shaped reagent hole is arranged in the sealed end of the holding barrel, a fluorescent detection module is releasably arranged above the gene amplification module and comprises a flip bottom plate and a cover arranged on the flip bottom plate, the flip bottom plate is provided with a through hole, a transparent glass plate is arranged in the through hole, a rotary drive is fixed to the top of the flip bottom plate through a fixing support, the output end of the rotary drive is connected to a cylinder below, spacing is reserved between the bottom of the cylinder and the flip bottom plate, and the cylinder follows when the rotary drive rotates.

Description

technical field [0001] The invention relates to a PCR gene amplification detector, in particular to a real-time fluorescence quantitative PCR gene amplification detector. Background technique [0002] PCR (Polymerase Chain Reaction) technology, translated into Chinese as polymerase chain reaction, is a technology for amplifying nucleic acids in vitro. The basic reactions of PCR include reactions using DNA as a template and reactions using mRNA as a template. PCR technology is to simulate the replication process of natural DNA (deoxyribonucleic acid) in the body. Taking amplified DNA as an example, the basic principle is to specifically amplify the enzymatic synthesis reaction of a DNA segment located between two known sequences in the presence of templates, primers, 4 dNTPs and thermostable DNA polymerases . [0003] The PCR reaction generally sets 20 to 40 cycles, and each cycle includes three steps of high-temperature denaturation, low-temperature annealing, and medium-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/68
CPCC12M41/10C12M41/12C12Q1/686C12Q2561/113C12Q2563/107C12Q2545/114C12Q2531/113
Inventor 杨松江
Owner 滨江华康(北京)生物科技有限公司
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