A kind of preparation method and application of Morus alba extract
A technology of Cortex Morus alba extract and Cortex Morus alba, applied in the field of medicine, can solve the problems of easy destruction of heat-sensitive active ingredients, low extraction yield of active ingredients, and difficulty in separation and purification, and achieve the regulation of adipocyte secreted factors and inflammatory factors. , Significantly improved curative effect and short extraction time
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Embodiment 1
[0026] Embodiment 1: the preparation method of Morus alba extract
[0027] (1) Raw material pretreatment: Fully dry the raw material of Morus alba, crush the raw material of Morus alba into powder with a pulverizer, pass through a 120-mesh sieve, pack it in a 1000-mesh gauze bag, put it into the reaction kettle, and add 5 times volume of distilled water for 0.5 hour to make the Morus Alba raw material fully absorb water and swell;
[0028] (2) Biological enzymolysis: after the Morus alba raw material in the step (1) fully absorbs water and swells, add the cellulase that dissolves fully with the same volume of the distilled water in the reactor, and the final concentration of the cellulase is 200U / mL, then oscillating enzymatic hydrolysis at 180rpm room temperature for 0.5h;
[0029] (3) Solvent extraction: after oscillating enzymolysis in the step (2) for 0.5-6 hours, continue to add ethanol in the reaction kettle, mix well, make the final concentration of ethanol be 60% by v...
Embodiment 2
[0031] Embodiment 2: the preparation method of Morus alba extract
[0032] (1) Raw material pretreatment: Fully dry the raw material of Morus alba, crush the raw material of Morus alba into powder with a pulverizer, pass through a 120-mesh sieve, pack it in a 2000-mesh gauze bag, put it into the reaction kettle, and add 2 times volume of distilled water for 2 hours to make the Morus alba raw material fully absorb water and swell;
[0033] (2) Biological enzymatic hydrolysis: after the Morus alba raw material in the step (1) fully absorbs water and swells, add the same volume of distilled water as the distilled water to dissolve the sufficient biological enzyme in the reaction kettle, and the final concentration of the biological enzyme is 300U / mL, the biological enzyme is cellulase, hemicellulase, xylanase, and pectinase configured in a ratio of 1:1:1:1, and then oscillating enzymolysis at 180rpm at room temperature for 6h;
[0034] (3) Solvent extraction: after oscillating ...
Embodiment 3
[0036] Embodiment 3: the preparation method of Morus alba extract
[0037] (1) Raw material pretreatment: fully dry the Morus alba raw material, crush the Morus alba raw material into powder with a pulverizer, pass through a 120-mesh sieve, pack it in a 500-mesh gauze bag, put it into the reaction kettle, and add 2 times volume of distilled water for 0.5 hour to make the Morus Alba raw material fully absorb water and swell;
[0038] (2) Biological enzymolysis: after the Morus alba raw material in the step (1) fully absorbs water and swells, add the same volume as the distilled water to dissolve the sufficient biological enzyme in the reaction kettle, and the final concentration of the biological enzyme is 50U / mL, the biological enzyme is configured by cellulase, hemicellulase, and xylanase in a ratio of 1:1:1, and then oscillating enzymolysis at 180rpm at room temperature for 0.5h;
[0039] (3) Solvent extraction: after oscillating enzymolysis for 0.5h in the step (2), conti...
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