Peripheral blood stem cell preserving fluid and preparation method thereof

A peripheral blood stem cell and preservation solution technology, applied in the field of peripheral blood stem cell preservation solution and its preparation, can solve the problems of low survival rate of peripheral blood stem cells, poor cell stability, and single nutrient composition, so as to improve survival rate and reduce damage , the effect of less dosage

Inactive Publication Date: 2017-09-15
魏方萌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, after peripheral blood stem cells are collected, it is necessary to add a preservation solution for cryopreservation. The existing peripheral blood stem cell

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A peripheral blood stem cell preservation solution, comprising the following components in parts by weight: 3 parts of human albumin, 2 parts of trehalose, 1 part of hydroxyethyl starch, 8 parts of nutrients (vitamin C, B vitamins, amino acid, alanine, sodium chloride, potassium chloride and sodium gluconate according to the mass ratio composition of 0.2:0.1:0.3:0.2:0.3:0.3:0.2), 1 part of dextran, 1 part of dimethyl sulfoxide ( The mass concentration is 5%) and appropriate amount of water.

Embodiment 2

[0026] A peripheral blood stem cell preservation solution, comprising the following components in parts by weight: 5 parts of human albumin, 3 parts of trehalose, 2 parts of hydroxyethyl starch, 10 parts of nutrients (vitamin C, B vitamins, taurine Acid, tyrosine, sodium chloride, potassium chloride and sodium gluconate are composed according to the mass ratio of 0.2:0.1:0.4:0.3:0.3:0.3:0.3), 2 parts of dextran, 2 parts of dimethyl sulfoxide (mass Concentration is 5%), 2 parts of 0.5wt% hyaluronic acid, 1 part of streptomycin and appropriate amount of water.

[0027] The preparation method of above-mentioned preservation solution:

[0028] (1) Dissolve nutrients, trehalose and hydroxyethyl starch in water in sequence according to the proportion, mix evenly, and keep the obtained solution at 37°C for later use;

[0029] (2) Cool the solution in step (1) to 26°C, add hyaluronic acid, mix well, and keep the obtained mixed solution at 26°C;

[0030] (3) Add dextran and human ser...

Embodiment 3

[0033] A peripheral blood stem cell preservation solution, comprising the following components in parts by weight: 6.5 parts of human albumin, 3.5 parts of trehalose, 2.5 parts of hydroxyethyl starch, 12 parts of nutrients (vitamin C, B vitamins, taurine acid and arginine 1:1 ratio, tyrosine, sodium chloride, potassium chloride and sodium gluconate according to the mass ratio composition of 0.3:0.2:0.5:0.4:0.3:0.4:0.5), 2.5 parts of dextran, 2.5 parts of dimethyl sulfoxide (mass concentration is 7%), 2.5 parts of 1wt% hyaluronic acid, 2 parts of streptomycin and appropriate amount of water.

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PUM

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Abstract

The invention relates to the technical field of stem cells and in particular relates to a peripheral blood stem cell preserving fluid. The peripheral blood stem cell preserving fluid comprises the following components in parts by weight: 3-8 parts of human serum albumin, 2-4 parts of trehalose, 1-3 parts of hydroxyethyl starch, 5-15 parts of nutrients, 1-3 parts of dextran, 1-3 parts of dimethyl sulfoxide and a proper amount of water. The preserving liquid is used to preserve the peripheral blood stem cells, so that the stem cells keep relatively good stability and the survival rates of the stem cells are greatly increased (over 98%).

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a peripheral blood stem cell preservation solution and a preparation method thereof. Background technique [0002] Stem cell therapy is to culture stem cells with continuous self-reproduction ability and pluripotency potential in vitro, and then transplant them into damaged or defective tissues of patients, so as to realize the supplement and repair of damaged tissues. Autologous peripheral blood hematopoietic stem cell transplantation refers to chemotherapy plus granulocyte colony-stimulating factor mobilization for patients with certain malignant blood diseases to make the patient's own peripheral blood stem cells reach a certain value, and then collect the collected hematopoietic stem cells through a cell separator. Stem cells are cryopreserved, and then the patient is treated with radiotherapy and chemotherapy, and the cryopreserved hematopoietic stem cells are resuscitate...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 魏方萌
Owner 魏方萌
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