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Cultivation medium and cultivation method of Phlebopus portentosus

A technology of Dictyostelium phalanx and culture medium, which is applied in the field of cultivation of Dictiostelium phalaenopsis boletus, can solve the problem that the yield rate of Dictiostelium phelostelium is yet to be studied, the resources of Boletus phelostelium phalanx are reduced, and the Problems such as the low yield of Boletus spp.

Inactive Publication Date: 2017-09-15
东莞东阳光保健品研发有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of this fungus is mainly the artificial harvesting in the wild state, which cannot meet the growing needs of the society. In addition, artificial high-intensity harvesting has reduced the resources of Dictyostelium bruceae and damaged the ecological environment.
[0003] In the prior art, after the cultivars are cultivated for 40-60 days, the hyphae can cover the cultivation bottles or cultivation bags, and the cultivation time is relatively long
In addition, after harvesting a crop of Dictyostelium pallidus, the fruiting cycle is over, and no effective measures are taken to cultivate the cultivars for secondary fruiting, resulting in a low yield of Dictyostelium palnus
[0004] At present, how to improve the yield of Dictyostelium bursa is still to be studied

Method used

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  • Cultivation medium and cultivation method of Phlebopus portentosus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this example, the effect of the MS medium content in the cultivar medium and the MS medium content in sterile water was investigated.

[0029] Specific steps are as follows:

[0030] 1. Preparation of liquid strains

[0031] Liquid medium (i.e. MS medium, 1000ml): 200g potato, 20g glucose, 10g yeast powder, KH 2 PO 4 10g, MgSO 4 10g, the balance water.

[0032] Preparation steps:

[0033] 1) Peel the fresh potatoes, weigh them, cut them into thin slices, put them in a stainless steel pot, add an appropriate amount of water and boil for 30 minutes, take the filtrate, and weigh the glucose, yeast powder, KH 2 PO 4 , MgSO 4 , added to the filtrate, supplemented with water to 1000ml, fully dissolved to obtain a liquid medium. The liquid medium was divided into 500ml Erlenmeyer flasks, with a volume of 300ml per bottle, and sterilized at 121°C for 30 minutes.

[0034] 2) Cut the mother seed culture medium into 2-4mm cubes, inoculate into liquid culture medium, c...

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Abstract

The invention provides a cultivation medium, and a cultivation method of Phlebopus portentosus. The medium comprises a dry material and an MS medium accounting for 1-5% of the total mass of the dry material. The cultivation medium used to cultivate the Phlebopus portentosus effectively shortens the cultivation time and increases the cultivation efficiency. The method adopting a double fruiting cultivation manner further obtains a large amount of the Phlebopus portentosus, and is suitable for large-scale production.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to a method for cultivating Dictyostelium pheasatum boletus. Background technique [0002] Phlebopus portentosus belongs to the family Boletaceae of the order Boletus and belongs to the genus Phlebopus. The earliest description was found in Sri Lanka, and now it is distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia and New Zealand. It is mainly distributed in Guangxi, Hainan and Yunnan in China. At present, the utilization method of the fungus is mainly the artificial harvesting in the wild state, which cannot meet the growing needs of the society. In addition, the artificial high-intensity harvesting reduces the resources of Dictyostelium bruceae and destroys the ecological environment. [0003] In the prior art, the hyphae of the cultivars can be covered with cultivation bottles or cultivation bags only after 40-60 days ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04C12N1/14C12R1/645
CPCC05B7/00C05G5/40C12N1/14C05D5/00C05F11/00C05F11/08
Inventor 胡生华向婷朱志钢
Owner 东莞东阳光保健品研发有限公司
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