Method of producing goat anti-human serum amyloid protein A antiserum
A technology of amyloid and human serum, applied in the field of bioengineering, can solve the problems of low level, high amount of antigen, and slow antibody production speed, and achieve the effect of fast speed, small amount of antigen, and high antibody level
Inactive Publication Date: 2017-09-15
海奥斯生物科技镇江有限公司
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AI Technical Summary
Problems solved by technology
Although the current production methods can produce a certain amount of antibodies, the antibody production speed is slow, the level is low, and the amount of antigen is high
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0028] Embodiment 1: a kind of method of producing goat anti-human serum amyloid A antiserum, the method comprises the following steps:
[0029] (1) Antigen preparation: Human serum amyloid A antigen was selected and emulsified with Freund's adjuvant before injection to obtain antigen emulsion; the following Freund's adjuvant used for emulsification before the first injection was complete Freund's adjuvant, and the second The Freund's adjuvant used for emulsification before the first, third, fourth, fifth, sixth and seventh injections is incomplete Freund's adjuvant;
[0030] (2) The first injection: Inject the sheep with the human serum amyloid A (SAA) antigen emulsion prepared by the method in step (1); the injection sites are 1 point in the hindfoot fossa and 1 point in the left and right submandibular lymph nodes , 2 points around the armpit, 3 points around the femoral lymph nodes, 4 points on the left and right sides of the back spine, a total of 22 points; the dose for ...
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The invention discloses a method of producing goat anti-human serum amyloid protein A antiserum. The method comprises the following steps: antigen preparation: selecting a human serum amyloid protein A antigen and emulsifying the human serum amyloid protein A antigen with a freund's adjuvant before injection to obtain an antigen emulsion; performing second-time injection 14 days after first-time injection; performing third-time injection 12 days after second-time injection; performing fourth-time injection 10 days after third-time injection; performing fifth-time injection 8 days after fourth-time injection; performing sixth-time injection 8 days after fifth-time injection; performing seventh-time injection 8 days after sixth-time injection; performing eighth-time injection 8 days after seventh-time injection; performing serum titer detection: collecting blood 8-10 days after injection, separating serum and detecting double-expanded titer thereof; and selecting 22 part points for the first time and selecting 8-16 part points for the second to eighth times for injection. By adopting the method disclosed by the invention, the cost is low and the process is simple; an antibody can be stably produced, and the speed of generating the antibody is high, the antibody level is high, the dosage of the antigen is small, and the immune period can be also shortened.
Description
technical field [0001] The invention belongs to the field of bioengineering, in particular to a method for producing goat anti-human serum amyloid A antiserum. Background technique [0002] Human serum amyloid A (SAA) is an acute phase protein and binds plasma high-density lipoprotein (HDL). [0003] Antiserum is serum containing a certain type of antibody molecule with specific immune function, generally animal serum prepared after animals are artificially injected with a certain type of antigen. Whether an antigen can cause antibody production depends on whether there is an antigenic determinant on the surface of the antigen molecule on the one hand, and on the other hand depends on the immune status of the body. When the above two conditions are met, antibody production will follow the antibody General law of generation - first reaction and second reaction. There are many kinds of antigens, including natural protein antigens and cellular antigens, synthetic antigens and...
Claims
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IPC IPC(8): C07K16/18C07K16/06
Inventor 袁金城王耀陈锁福王成新
Owner 海奥斯生物科技镇江有限公司