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Augmented triple-test cross design QTL mapping method based on RIL population

A mapping method and basic population technology, applied in the field of plant quantitative genetics, can solve problems such as inability to clearly compare pure genetic effects, large differences in results, and mixed genetic effects

Inactive Publication Date: 2017-09-15
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

A large number of articles on QTL mapping under NCIII genetic mating design have been published. Different materials or different QTL mapping methods, such as interval mapping, compound interval mapping, multi-interval mapping, Bayesian regression, etc. , the results are quite different, although these results can complement each other, but because the genetic effects of each site are mixed, it is impossible to clearly compare the pure genetic effects of each site

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  • Augmented triple-test cross design QTL mapping method based on RIL population
  • Augmented triple-test cross design QTL mapping method based on RIL population
  • Augmented triple-test cross design QTL mapping method based on RIL population

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Embodiment Construction

[0031] The present invention is a QTL mapping method based on extended three-way test cross design (aTTC), and its steps are as follows:

[0032] Step A: Construct a data set conversion model of the population.

[0033] According to the aTTC design proposed by Liu Dingfu, with RIL, BC or F 2 The group is the basic group. The data set conversion formula included in the aTTC design is: Z 1i =L 1i +L 2i ,Z 2i =L 1i -L 2i ,Z 3i =L 1i +L 2i -2L 3i ,Z 4i =L 1i +L 2i -L 4i ,Z 5i =L 1i +L 2i +L 3i And Z 6i = 2L 3i -L 4i . When designing aTTC with RIL as the basic group, only Z 1i ,Z 2i And Z 4i Then the homozygous genetic effects of each site can be analyzed. At this time, only need to use L in the above formula 1i , L 2i And L 4i . In F ∞ Under the standard, RIL, L 1i , L 2i And L 4i Each individual (RIL, L 1i , L 2i And L 4i The genetic expected values ​​of different genotypes of) are shown in Table 1 to Table 4 (He Xiaohong et al., 2012). Since each point of the RIL group is homozygou...

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Abstract

A QTL mapping method based on augmented TTC design is provided, which is based on recombinant inbred line group as the basic group; based on the genetic expectation values of RIL, L1i, L2i and L4i under the F infinity scale, the genetic expectation values s of Z1i, Z2i and Z4i under RIL-based aTTC are deduced; and then according to the genetic expectation values of Z1i, Z2i and Z4i, the phenotype value representation formulas of Z1i, Z2i and Z4i are simplified. Then the Bayesian method was used to carry out two-dimensional scanning of the whole genome to obtain various pure-effect and epistatic effects of each QTL locus in the genetic model in two steps. Compared with the TTC design, the aTTC design QTL mapping method can reduce the workload of a one-time hybridization, which eliminates the process of basic group RIL and F1 hybrids and constructing the population of L3i population. For the field work, manpower and resources are greatly saved; but the statistical efficiency of QTL mapping and estimation of genetic effects are not reduced.

Description

Technical field [0001] The invention belongs to the field of plant quantitative genetics, and specifically relates to an augmented triple-test cross design (aTTC, augmented triple-test crossdesign) QTL mapping method in which the basic population is a recombinant inbred line (RIL) population. Background technique [0002] The use of heterosis can significantly increase crop yields. NCIII design (north Carolina design III) and TTC design (triple-test cross design) are two excellent genetic designs for studying heterosis, which are widely used in QTL analysis of crop heterosis. The NCIII design includes the basic mapping population (such as recombinant inbred line RIL, doubled haploid DH, backcross population BC or F 2 Group, etc.) and the backcross group backcrossed with the two parents separately (denoted as L 1i , L 2i ). The TTC design is based on the NCIII design, adding a basic mapping group and F 1 Testcross group (denoted as L 3i ). The creation of NCIII or TTC design make...

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Application Information

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IPC IPC(8): G06F19/20
CPCG16B25/00
Inventor 李兰芝孙丛苇
Owner HUNAN AGRICULTURAL UNIV
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