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Vaccines based on hepatitis b core antigens

A technology of hepatitis B and core antigen, which is applied in the field of vaccines based on hepatitis B core antigen, and can solve the problems that influenza vaccines have not yet been developed

Inactive Publication Date: 2017-09-26
IQUR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the variability of these two proteins, a broad-spectrum, long-acting influenza vaccine has not been developed so far

Method used

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  • Vaccines based on hepatitis b core antigens
  • Vaccines based on hepatitis b core antigens
  • Vaccines based on hepatitis b core antigens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0206] Materials and Methods:

[0207] VLP sequence:

[0208] in BL21 E. coli A tandem core of inserts from influenza virus conserved proteins contained in MIRs was prepared in . The sequence corresponding to the insert belongs to the region of the extracellular domain of influenza virus matrix protein 2 (M2e). It spans 24 amino acids encoding the N-terminal external sequence of the known M2 protein ( Figure 5 ); MSLLTEVETPIRNEWG C R C NGSSD (SEQ ID NO: 6). The wild-type sequence was modified to replace cysteine ​​residues (which affect VLP formation) at positions 17 and 19 (underlined) with serine residues. The final insert contained three variants of this sequence. The first version is the general M2e consensus sequence, except that the cysteine ​​residues at positions 17 and 19 have been replaced by serine (SEQ ID NO: 9). The second (SEQ ID NO: 8) and third (SEQ ID NO: 10) are mutated versions of the general sequence, which correspond to the most common variants...

Embodiment 2

[0238] Materials and Methods:

[0239] VLP sequence:

[0240] in BL21 E. coli A tandem core of inserts from influenza virus conserved proteins contained in MIRs was prepared in . The sequence corresponding to said insert belongs to the stem region of the influenza virus hemagglutinin HA2 protein domain. It spans nucleic acid encoding known domains; loop B, helix C, helix CD, and helix D of the HA monomer ( Figure 10 ). The sequence spans amino acids (aa) 403-474 of the HA protein isolated from influenza A virus H1N1 / Lux / 09. There are other constructs comprising the HA stem insert sequence, these are described in Table 4 below.

[0241] Table 4. Table of alternative VLPs generated by changes in the insert sequence.

[0242]

[0243] The amino acid sequence of the tandem core with the HA stem influenza insert (encoded by the single letter aa) is shown below. Amino acids from the tandem core are bolded, while amino acids from influenza HA are underlined:

[0244]...

Embodiment 3

[0262] Materials and Methods:

[0263] VLP sequence:

[0264] in BL21 E. coli A tandem core containing insertions from conserved proteins of influenza virus within two major insertion regions (MIRs) was prepared in (Fig. 15). The sequence corresponding to the first insert belongs to the stem region of the influenza virus hemagglutinin HA2 protein domain. It spans nucleic acid encoding known domains; loop B, helix C, helix CD, and helix D of the HA2 monomer ( Figure 10 ). The sequence spans amino acids (aa) 403-474 of the HA protein isolated from influenza A virus H1N1 / Lux / 09. The sequence corresponding to the second insert belongs to the region of the extracellular domain of influenza virus matrix protein 2 (M2e). It spans 24 amino acids encoding the N-terminal external sequence of the known M2 protein; MSLLTEVETPIRNEWG C R C NGSSD (SEQ ID NO: 6) ( Figure 5 ). The wild-type sequence was modified to replace cysteine ​​residues (which affect VLP formation) at posit...

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Abstract

The invention provides a protein comprising hepatitis B core antigen (HBcAg) and influenza virus A surface polypeptide M2 or an immunogenic fragment thereof. The invention also provides a protein comprising hepatitis B core antigen (HBcAg) and influenza virus hemagglutinin (HA) or an immunogenic fragment thereof. The invention also provides particles formed from the proteins, nucleic acid molecules encoding the proteins, processes for producing the proteins, pharmaceutical compositions containing the proteins and use of the proteins to induce an immune response in a subject.

Description

technical field [0001] The present invention relates to a protein comprising hepatitis B core antigen (HBcAg) and influenza virus A surface polypeptide M2 ​​or its immunogenic fragment. The invention also relates to particles formed from said protein, nucleic acid molecules encoding said protein, methods of preparing said protein, pharmaceutical compositions comprising said protein and the use of said protein for inducing an immune response in a subject. Background technique [0002] The Hepatitis B virus core (HBc) protein has a somewhat unique structure consisting of two antiparallel alpha helices forming a characteristic "spike" structure. Two HBc molecules then dimerize spontaneously to form a double spike. The twin spikes are the building blocks of virus-like particles (VLPs). VLPs are attractive vaccine systems because of their highly repetitive nature to deliver multiple copies of an antigen. Further, the absence of viral nucleic acid makes them safe vectors. HBc ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00A61K39/145C12N7/00C12N15/44A61P31/16
CPCA61K39/12C07K14/005A61K2039/627A61K2039/70A61K2039/5258A61K2039/5256C07K2319/00C12N2730/10123C12N2760/16134A61P31/16A61P31/20A61P37/04Y02E60/10H01M4/505H01M4/525H01M4/625H01M10/0525
Inventor 迈克尔·安东尼·惠兰亚历杭德罗·拉米雷斯·塞瓦略斯文森索·克雷申特
Owner IQUR
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