Microbe culture solution treatment method applicable to MALDI-TOF detection and rapid identification method

A liquid treatment and microbial technology, applied in the field of microbial detection, can solve the problems of long identification process time, etc., achieve the effect of low cost of treatment reagents, simple treatment process, and reduce abuse

Inactive Publication Date: 2017-09-29
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients with bloodstream infection are generally in serious condition. The identification process of microorganisms in the traditional blood culture positive bottle takes a long time. Often doctors need to give patients empirical medication before the identification results come out, so the probability of wrong medication is high.

Method used

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  • Microbe culture solution treatment method applicable to MALDI-TOF detection and rapid identification method
  • Microbe culture solution treatment method applicable to MALDI-TOF detection and rapid identification method
  • Microbe culture solution treatment method applicable to MALDI-TOF detection and rapid identification method

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Embodiment 1

[0031] Embodiment 1, the method for rapid identification of microorganisms in blood culture positive bottles

[0032] The method for rapid identification of microorganisms in blood culture positive bottles, the specific steps are as follows:

[0033] (1) Collection of blood culture-positive bacterial suspensions: After receiving the blood culture bottles and putting them into the Bactec FX automatic blood culture system for detection, the positive blood was taken out after the instrument reported positive For the culture bottle, sterilize the mouth of the bottle in a biological safety cabinet, mix well, take 1mL of the culture solution in the bottle with a syringe, inject it into a 1.5mL EP tube to make a bacterial suspension, and wait for treatment;

[0034] (2) Bacterial suspension treatment: under sterile conditions (biological safety cabinet), add 200 μL of glacial acetic acid solution with a volume fraction of 100% to the EP tube containing the bacterial suspension, vorte...

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Abstract

The invention relates to a microbe culture solution treatment method applicable to MALDI-TOF detection and a rapid identification method. The treatment method comprises the specific steps of adding a hemoculture positive bacterium suspension into glacial acetic acid to lyse red blood cells, carrying out centrifugation to remove supernatant, adding a 10% (volume fraction) glacial acetic acid solution into precipitates to lyse red blood cells, carrying out uniform mixing, then, carrying out centrifugation again to remove supernatant, thereby obtaining precipitates, i.e., a substance to be assayed. The treatment steps are simple, and the reagent cost is low. The invention further discloses a rapid identification method for microbes in a hemoculture positive bottle. According to the rapid identification method, the consumed time is short, clinical reliable identification results can be offered in a short time, the clinical use of antibiotics can be better instructed, and the abuse of the antibiotics is reduced.

Description

technical field [0001] The invention belongs to the field of microorganism detection, relates to a method for treating microorganism culture liquid suitable for MALDI-TOF detection, and also relates to a rapid identification method for microorganisms in positive blood culture bottles. Background technique [0002] Bloodstream infection is a serious systemic infectious disease. The microorganisms in the hospital are transient, indirect or persistent in the circulating blood, causing damage to all organs of the body, especially heart valves and joints. In severe cases, it can lead to Shock, multiple organ failure, disseminated intravascular coagulation, and even death. Blood cultures are currently the gold standard for diagnosing bloodstream infections. Blood culture is to inoculate freshly isolated blood specimens into one or more culture bottles or culture tubes, and make bacteria or fungi with high nutritional requirements grow and reproduce under certain conditions such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/62
CPCG01N1/28G01N27/62
Inventor 李涛刘飞蒲晓允张立群蒋栋能项贵明黄微微
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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