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Chicory root tobacco flavor as well as preparation method and application thereof

A technology for tobacco spices and chicory, which is applied in the fields of cigarette flavors and tobacco flavors, to achieve the effect of easy operation, simple process, and rich smoke aroma

Active Publication Date: 2017-10-03
CHINA TOBACCO YUNAN NEW MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no relevant report on the method of fermenting and extracting the nutrients and aroma of chicory using microorganisms, and applying it to cigarettes as flavoring and fragrance for tobacco

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the chicory root tobacco spice of the present embodiment comprises the following steps:

[0024] (1) Take Bacillus subtilis CGMCC NO.4091 and carry out activation culture in LB liquid medium to obtain Bacillus subtilis activated bacteria liquid; the culture conditions for the activation of the bacteria are 37°C, 120r / min shaking culture, and the culture time is 12h;

[0025] (2) Take 100 g of crushed chicory root powder, add 15 times the amount of water, and sterilize at 121°C for 30 minutes to make a sample to be fermented;

[0026] (3) Use Bacillus subtilis CGMCC NO.4091 to ferment it, inoculate the activated bacterial liquid in step (1) into the sample to be fermented, the inoculation amount of the strain is 1%, and the fermentation conditions are: rotation speed 120rmp, fermentation temperature 37°C, fermentation time 12h;

[0027] (4) After the fermentation broth is filtered and centrifuged, the supernatant is taken, concentrated under re...

Embodiment 2

[0031] The preparation method of the chicory root tobacco spice of the present embodiment comprises the following steps:

[0032] (1) Take Bacillus subtilis CGMCC NO.4091 and carry out activation culture in LB liquid medium to obtain Bacillus subtilis activated bacteria liquid; the culture conditions for the activation of the bacteria are 37°C, 120r / min shaking culture, and the culture time is 12h;

[0033] (2) Take 100g of crushed chicory root powder, add 20 times the amount of water, and sterilize at 121°C for 30 minutes to make a sample to be fermented;

[0034] (3) Use Bacillus subtilis CGMCC NO.4091 to ferment it, inoculate the activated bacterial liquid in step (1) into the sample to be fermented, the inoculum amount of the strain is 2%, and the fermentation conditions are: rotation speed 120rmp, fermentation temperature 37°C, fermentation time 12h;

[0035] (4) After the fermentation broth is filtered and centrifuged, the supernatant is taken, concentrated under reduce...

Embodiment 3

[0039] The preparation method of the chicory root tobacco spice of the present embodiment comprises the following steps:

[0040] (1) Take Bacillus subtilis CGMCC NO.4091 and carry out activation culture in LB liquid medium to obtain Bacillus subtilis activated bacteria liquid; the culture conditions for the activation of the bacteria are 33°C, 160r / min shaking culture, and the culture time is 18h;

[0041] (2) Take 100 g of crushed chicory root powder, add 15 times the amount of water, and sterilize at 121°C for 30 minutes to make a sample to be fermented;

[0042] (3) Use Bacillus subtilis CGMCC NO.4091 to ferment it, inoculate the activated bacterial liquid in step (1) into the sample to be fermented, the inoculation amount of the strain is 1%, and the fermentation conditions are: rotation speed 160rmp, fermentation temperature 33°C, fermentation time 18h;

[0043] (4) After the fermentation broth is filtered and centrifuged, the supernatant is taken, concentrated under re...

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Abstract

The invention disclose a chicory root tobacco flavor as well as a preparation method and application thereof. The needed tobacco flavor is obtained by the following steps: crushing chicory roots, adding water with 10-20 times the amount of the chicory roots, performing sterilization under a high-temperature and high-pressure condition to prepare a fermentation medium, performing fermentation with Bacillus subtilis (CGMCC NO.4091) on the fermentation medium, and performing filtration and concentration after fermentation. The chicory root tobacco flavor is used as a base uniformly sprayed on cut tobaccos in use. According to the invention, the Bacillus subtilis is initiatively used for fermenting the chicory roots to prepare the tobacco flavor; active ingredients and a peculiar smell in the chicory roots, after compounded with the cut tobaccos, can effectively improve the cigarette quality, correct cigarette smoking smell, and improve the aroma quality of cigarettes; the method is suitable for promotion and application, and is of positive significance of promoting healthy development of the cigarette-making industry.

Description

technical field [0001] The invention relates to a flavoring for cigarettes, in particular to a flavoring for cigarettes prepared from chicory root. At the same time, the invention also relates to the preparation method and application of the spice for cigarettes, belonging to the technical field of tobacco additives. Background technique [0002] Flavors and fragrances play a key role in the taste of cigarette products. At present, most of the spices are obtained through chemical synthesis, but chemically synthesized spices are not only expensive and difficult to synthesize, but also have certain impacts on the environment and health. The spices directly extracted from natural animals and plants have problems such as high extraction cost and limited raw materials. The spices prepared by fermenting plant materials with microbial technology belong to the level of natural spices, and while improving product quality, resources can be saved and costs can be reduced. [0003] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/00C12P1/04A24B15/20A24B3/12C12R1/125
CPCA24B3/12A24B15/20C11B9/0003C12P1/04C12N1/205C12R2001/15
Inventor 杨培香段焰青徐世涛陆廷伟李月菊王夸平刘秀明韩智强
Owner CHINA TOBACCO YUNAN NEW MATERIAL
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