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Soybean seed disinfection method for tissue culture

A technology of soybean seed and tissue culture, which is applied in the direction of seed immunity, horticultural methods, botanical equipment and methods, etc., can solve the problems of poor water permeability of soybean seed coat, easy contamination of explants, failure of tissue culture, etc. Effects of Tainted Abilities

Inactive Publication Date: 2017-10-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the poor water permeability of soybean seed coats and the differences in water permeability of seed coats among different varieties, it is easy to happen that soybean seeds are not thoroughly disinfected during the disinfection process, and the explants are easily contaminated, resulting in tissue culture failure.

Method used

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  • Soybean seed disinfection method for tissue culture

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Embodiment Construction

[0011] The present invention will be further described below. It should be noted that the following embodiments are based on the technical solution and give detailed implementation and specific operation procedures, but the protection scope of the present invention is not limited to this embodiment.

[0012] An improved method for sterilizing soybean seeds for tissue culture, including the following steps:

[0013] S1 First, treat and disinfect the soybean seeds for tissue culture with 70% alcohol for 1 min;

[0014] S2 is soaked in 0.2% sulfuric acid for 5 minutes, and then disinfected with 1% sodium hypochlorite for 15 minutes;

[0015] S3 is rinsed with sterile water, and finally soaked in sterile water.

[0016] Further, in step S3, rinse with sterile water for 4 to 5 times, and finally soak in sterile water for 17 to 20 hours.

[0017] In order to further illustrate the technical effect of the method of the present invention, the comparative experimental data are given as shown in T...

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Abstract

The invention discloses a soybean seed disinfection method for tissue culture. Soybean seeds are treated by 70% alcohol for 1 minute, then treated by 0.2% sulfuric acid for 5 minutes, disinfected by 1% sodium chlorate for 15 minutes, flushed by sterile water for 4-5 times and finally soaked in the sterile water for 17-20 hours. The soybean seeds are treated by the 0.2% sulfuric acid, poor water permeability can be effectively broken, and the contamination resistance of the soybean seeds is improved.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to an improved method for sterilizing soybean seeds for tissue culture. Background technique [0002] Soybean is an important economic crop. In recent years, with the development of plant transgenic technology, soybean tissue culture has been extensively studied. Commonly used explants in soybean tissue culture include cotyledon nodes and germ tips. Therefore, obtaining sterile infected explants is the key to successful soybean tissue culture. However, due to the poor water permeability of soybean seed coats and differences in the water permeability of seed coats between different varieties, it is prone to incomplete sterilization of soybean seeds, and the explants are easily contaminated, leading to failure of tissue culture. Summary of the invention [0003] In view of the shortcomings of the prior art, the present invention aims to provide an improved method for sterilizing soybean seeds...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08A01H4/00
CPCA01C1/08A01H4/00
Inventor 李军刘立功宋希云孙吉同
Owner QINGDAO AGRI UNIV