Differential phase contrast microscope imaging method based on half-ring-shaped LED lighting

Abstract

The present invention discloses a differential phase contrast microscope imaging method based on half-ring-shaped LED lighting. Firstly weak object approximation is used, and an optical transfer function expression of a weak object in a partial coherent illumination imaging system is deduced. A phase transfer function of differential phase contrast imaging in half-ring-shaped illumination is calculated. Two ring-shaped illumination patterns in different directions are orderly displayed on an LED array, and a synchronous triggering signal is generated and sent to a camera. The ring-shaped illumination pattern changes are changed, a synchronous triggering signal is generated and sent to the camera, in triggering signals corresponding to the ring-shaped illumination patterns in different directions, the camera collects a series of intensity images. Differential phase contrast images in different directions can be calculated by a differential phase contrast imaging calculation formula, and thus the differential phase contrast imaging can be realized in up, down, left and right directions and all different directions. According to the method, the low frequency of a low phase in a differential phase contrast image can be improved, thus the concave and convex sense of the differential phase contrast image is strong, and the imaging quality of the image is enhanced.

Description

technical field [0001] The invention belongs to optical microscopic measurement and imaging technology, in particular to a differential phase contrast microscopic imaging method based on semi-circular LED lighting. Background technique [0002] In the field of biomedical microscopic imaging, most living cells and unstained biological specimens are colorless and transparent. This is because the refractive index and thickness of the fine structures in each part of the cell are different. When light waves pass through, the wavelength and The amplitude does not change, only the phase, but this phase difference cannot be observed by the human eye. This requires some chemical or biological means to stain the cells to make them visible under the microscope. Because different substances in biological cells have different absorption rates for colored dyes, the internal structure of biological cells can be seen under the microscope and show different biological characteristics (Gong ...

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Problems solved by technology

Although this method can achieve differential phase contrast imaging based on LED arrays, the imaging effect of the phase low-frequency components in differential phase contrast images based on traditional semicircular illumination apertures still needs to be improved. here comes the difficulty

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