Nerve cell population

A technology of nerve cells and neural stem cells, applied in the field of nerve cell populations, to achieve the effects of enhancing activity, simple preparation methods, and saving time and energy

Inactive Publication Date: 2017-10-24
温州市鹿城区中津先进科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The culture methods of neural stem cells known in the art (due to the a...

Method used

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Embodiment Construction

[0018] Below by specific embodiment the present invention is described in further detail.

[0019] The methods used in the embodiments of the present invention include nerve tissue preservation solution, nerve tissue washing solution, nerve tissue decomposition solution, cell washing solution, primary cell culture solution, cell digestion solution, subculture cell culture solution, cell cryopreservation solution, etc. Wherein, the secondary cell culture medium is a serum-containing growth medium for neural stem cells, which consists of 1000ml basal medium, 50ug / ml fetal bovine serum, 10ng / ml bFGF, 10ng / ml EGF, 0.5mmol / ml ml glutamine, 0.5mmol / ml sodium pyruvate mixed composition. The secondary cell culture medium is the obtained neural stem cell adherence culture medium, which enables the neural stem cells to adhere to the wall and have stronger activity. The nerve tissue preservation solution is a culture medium containing one or more of RPMI-1640, DMEM, a-MEM and DMEM / F12 c...

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Abstract

The neural cell population of the present invention is characterized in that in the presence of F and FGF-2, the cells are in an adherent monolayer culture, and at least 80% of the cells are symmetrically divided neural stem cells, No more than 1% of the cells express markers of mature astrocytes, neurons, or oligodendrocytes, and they express the markers RC2, 3CB2, and BLBP, and neural stem cells in said population are also characterized in that they express At least one of the proteins: GLAST, Pax‑6, neural progenitor cell markers stem protein or vimentin, LewisX antigen, Musashi‑l or convexin. The preparation method of the invention is simple and easy to operate.

Description

technical field [0001] The present invention relates to neural stem cells and culture conditions and methods for culturing neural stem cells (NS cells or NSCs). In particular, a population of neural cells is provided. Background technique [0002] Although neural stem cells have been said to be isolated from a variety of sources in vitro, it has so far not been possible to expand cells in large-scale culture in a symmetrically dividing, undifferentiated state. Long-term expansion of cells in this state is highly desirable from both an experimental and a therapeutic point of view. The resulting pure neural stem cell population was capable of directed differentiation into three cell types: neurons, astrocytes, and oligodendrocytes. [0003] One known method of culturing neural stem cells (albeit as a small fraction of a heterogeneous cell population) is the neurosphere system. This system consists of serially passaged heterogeneous aggregates of cells, only a small fraction...

Claims

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Application Information

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IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2500/30C12N2500/32C12N2500/84C12N2501/11C12N2501/115
Inventor 王振宇
Owner 温州市鹿城区中津先进科技研究院
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