Primer group and kit for detecting and identifying clonorchis sinensis and/or haplorchis taichui

A technology for Clonorchis sinensis and Mononorchis sinensis, which is applied in the field of primer sets and kits for detecting and identifying Clonorchis sinensis and/or Mononorchis sinensis, and can solve the problem that PCR detection primers and methods are not suitable for clinical diagnosis and There are problems such as deviation and low efficiency in disease monitoring work and identification test results, so as to reduce the cost of detection and identification, improve the efficiency of detection and identification, and reduce the false negative rate.
CN107299145AInactive Publication Date: 2017-10-27GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2017-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of biologics, and particularly relates to a primer group and a kit for detecting and identifying clonorchis sinensis and / or haplorchis taichui. The primer group is selected from a nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.4. The primer group has the advantages of being good in specificity and high in amplification efficiency, primer dimer, non-specific amplification and cross reactions are avoided in the PCR (Polymerase Chain Reaction) amplification process, influence of non-target products to results is avoided, moreover, the amplification efficiency is high, and detection signals are intense.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a primer set and a kit for detecting and identifying Clonorchis sinensis and / or Monorches sinensis. Background technique

[0002] Clonorchis sinensis (Clonorchis sinensis) parasitizes the bile ducts in the human liver and can cause clonorchiasis and even cholangiocarcinoma. The occurrence and prevalence of Clonorchiasis sinensis are mainly related to the eating habits of people who prefer to eat raw freshwater fish and other foods. According to the survey, in Guangxi, where clonorchiasis is prevalent seriously, the average infection rate of Clonorchis sinensis among the population is 9.76%; among them, the population infection rate in individual endemic counties even exceeds 20%. Haplorchis taichui is a fluke of the family Heteromorphidae that lives in the intestinal tract. Monoorchis fanicum is commonly infected in people in Clonorchis endemic areas in Guangxi and other places. A...

Claims

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