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A method for the ultrasensitive detection of mercury ions based on the t-hg(ii)-t structure-based agncs probe

A technology for sensitive detection of mercury ions, applied in the field of biosensing, can solve the problems of low detection accuracy, narrow detection linearity, and high detection limit of mercury ions, and achieve the effect of wide detection concentration range, low detection limit, and high accuracy

Active Publication Date: 2019-11-19
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest defect of this type of method is that the detection accuracy of mercury ions by fluorescence quenching is low. In addition, fluorescent probes labeled with fluorescein are required, which are costly, complex to synthesize, high detection limits, and narrow detection linearity.

Method used

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  • A method for the ultrasensitive detection of mercury ions based on the t-hg(ii)-t structure-based agncs probe
  • A method for the ultrasensitive detection of mercury ions based on the t-hg(ii)-t structure-based agncs probe
  • A method for the ultrasensitive detection of mercury ions based on the t-hg(ii)-t structure-based agncs probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The DNA1 sequence number used is: 5'-TTTTTTCCCCCTAATTCCCAAAGCTCGACGGATT-3';

[0036] The DNA2 sequence number used is: 5'-AATCCGTCGAGCAGGGGAAG GGGAAGGGTTTTTT-3';

[0037] The preparation method of DNA1-AgNCs, the steps are as follows:

[0038] Take 33 μL of DNA1 (100 μM), and dissolve DNA1 with pH=7.4, 10 mM phosphate buffer (the DNA1 solution that has been centrifuged before use is stored in a refrigerator at 4°C). Next, mix 160 μL of phosphate buffer (20 mM, pH=7.0) with the above 33 μL DNA1, and add 13.2 μL of AgNO to the mixed solution. 3 Aqueous solution (1.5mM), the above-mentioned whole mixed solution was continuously shaken and mixed with a mixer for 15min (1000r / min) at room temperature, and then 13.2μL of freshly prepared NaBH 4 (1.5mM) solution was quickly added to the above mixed solution, and the whole solution was mixed for 30s, and then the whole solution was placed in a constant temperature water bath at 25°C for 2h. fluorescence.

[0039] The DNA1 / D...

Embodiment 2

[0052] The operation steps and experimental conditions were the same as in Example 1, except that the sequence number of DNA2 was replaced with 5'-AATCCGTCGAGCAGGGGAAGGGGAAGGGTTTTTTTCGACGACGACGACGACGACGACGACGACGA-3'.

[0053] from Figure 5 It can be seen that in the concentration range of 0.00002nM~1.6nM, Hg 2+ It has a certain linear relationship with the corresponding response signal.

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Abstract

The invention a method for performing supersensitive detection on mercury ions by using a T-Hg(II)-T structure-based AgNCs probe. The method comprises the following steps: synthesizing a siliver nano-cluster (DNA1-AgNCs) by taking DNA1 as a template, performing hybrid combination on the DNA1-AgNCs and DNA2 to obtain a DNA1 / DNA2-AgNCs fluorescent probe, and detecting the Hg(II) concentration of the solution by utilizing the fluorescence change of the DNA1 / DNA2-AgNCs fluorescent probe. The method has the advantages of label-free property, strong signal, high specificity, high sensitivity, wide detection concentration range, low detection limit and the like.

Description

technical field [0001] The present invention relates to a Hg 2+ A detection method, in particular to a DNA-based synthetic DNA1 / DNA2-AgNCs fluorescent probe as a template, and using the DNA1 / DNA2-AgNCs fluorescent probe to detect Hg 2+ The invention relates to a method for specific fluorescence detection, which belongs to the technical field of biosensing. Background technique [0002] Heavy metal mercury ions widely exist in nature in the form of inorganic salts and organic complexes as well as metallic mercury. Mercury ions are easy to accumulate in organisms through the food chain, resulting in permanent damage to the brain, liver and central nervous system. The U.S. Environmental Protection Agency (EPA) strictly defines the concentration of mercury ions in drinking water to be ≤10nM. At present, the trace detection of metallic mercury ions has become an urgent problem. The traditional and reliable detection methods of metallic mercury ions are mainly based on atomic a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/65
CPCC09K11/65G01N21/6428
Inventor 邓春艳丁亚林
Owner CENT SOUTH UNIV
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