A drug for parenteral nutrition-related liver disease

A parenteral nutrition and correlation technology, applied in the direction of introducing foreign genetic materials, drug combinations, viruses, etc. using vectors, can solve the problems of unclear pathogenic mechanism and unsatisfactory treatment effect, and achieve enhanced inhibitory effect and high safety. , the effect of high infection efficiency

Active Publication Date: 2020-11-10
SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0026] In summary, the prevention of PNALD currently mainly reduces the use time of parenteral nutrition, starts oral feeding as soon as possible, and uses ursodeoxycholic acid; the treatment mainly includes limiting the dose of fat emulsion, using fish oil fat emulsion and using mixed fat emulsion ; but because the pathogenic mechanism is not clear, the current treatment effect is not ideal

Method used

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  • A drug for parenteral nutrition-related liver disease
  • A drug for parenteral nutrition-related liver disease
  • A drug for parenteral nutrition-related liver disease

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0082] 293T cell culture

[0083] (1) Take out the frozen 293T cells from the refrigerator at -80°C, thaw them immediately in a 37°C water bath, and add them to 1ml of complete medium (DMEM high-glucose medium containing 10% fetal bovine serum) after they are completely thawed , 1000rpm, centrifugal 5min;

[0084] (2) Discard the supernatant, absorb 1 mL of complete medium with a 1 ml gun tip, blow off the cells, transfer to a 10 cm culture dish containing 12 mL of complete medium, and culture at 37°C, 5% CO2, and saturated humidity;

[0085] (3) On the second day, discard the culture medium, wash with 1×PBS for 3 times, replace the DMEM medium, and continue culturing. Observe the cell growth and cell status under an inverted phase-contrast microscope every day, and replace the medium every 2 days;

[0086] (4) When the cells are more than 90% confluent, suck off the medium, wash twice with 1×PBS, add 2 mL of 0.25% trypsin, and shake gently to make the trypsin completely cont...

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Abstract

The invention discloses a medicament for treating parenteral nutrition related liver disease. The medicament for treating the parenteral nutrition related liver disease is prepared by combining slow virus packaging plasmids, membrane expression plasmids and interference expression vector plasmids into a three-plasmid slow virus expression system, co-transfecting the three-plasmid slow virus expression system to packaging cells, and performing culturing and splitting. ShRNA (short hairpin Ribonucleic Acid) of a specific interference IRE1alpha gene is designed and packaged into slow viruses, thereby effectively importing BRL cells, achieving a stable interference effect of the IRE1alpha gene, and realizing relieving and treatment of the parenteral nutrition related liver disease.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a medicine for parenteral nutrition-related liver diseases. Background technique [0002] Parenteral nutrition (PN) has brought revolutionary care for newborns who cannot meet their growth through normal intestinal methods. However, there are many related problems in continuous application of PN for a long time (>2 weeks), and total parenteral nutrition-associated liver disease (PNALD) is one of the most common complications. With the application of long-term PN, the incidence of PNALD increases. PNALD has fatty liver and jaundice in different degrees. In severe cases, it can progress to liver cirrhosis, and eventually liver failure is life-threatening. Histological evidence of PNAC can be seen after 2 weeks of PN application, and varying degrees of fibrosis can be observed after 6 weeks. However, clinically, liver pathological examination is invasive and not a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7105A61P1/16C12N15/113C12N15/867
CPCA61K31/7105C12N15/113C12N15/86C12N2310/141C12N2740/15043
Inventor 朱雪萍汪健朱晓黎崔宁迅崔铭玲李洁陈小倩黄凤霞李之珩
Owner SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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