Method for preparing spirulina platensis active protein

A technology of Spirulina platensis and active protein is applied in the field of preparation of active protein of Spirulina platensis, can solve the problems of low yield of Arthrospira protein, low preparation efficiency and the like, and achieves improvement of immune function, simple operation and purity of the body. high effect

Inactive Publication Date: 2017-11-03
乐清瑞耀食品技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is low in production cost, simple and effective, and is suitable for preparing pharmaceutical grade Arthrospira protein, but the preparation efficiency is low, and the yield of Arthrospira protein is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation method of the active protein of Spirulina platensis comprises the extraction of crude protein, salting out, dialysis and purification, and the specific steps are:

[0016] 1) Extraction of crude protein: add distilled water to the algae powder according to the ratio of solid to liquid 1:3, filter with Buchner funnel, wash with deionized water for 3 times, add phosphate with a pH of 6.7 and a concentration of 0.008mol / L Buffer and active polypeptide, the weight-volume ratio of algae flour to phosphate buffer is 1:15, stir for 120min, freeze quickly at -20°C, then thaw at 4°C, freeze-thaw repeatedly 3 times, at 4°C, 12000rpm Centrifuge under high-speed refrigeration for 30 minutes, the supernatant is the crude protein solution, and the active protein belongs to the intracellular protein. First, the cell wall and cell membrane of the cell must be broken to dissolve it. The higher the degree of cell fragmentation, the higher the yield of active protein. The ...

Embodiment 2

[0023] The preparation method of spirulina platensis active protein, concrete steps are:

[0024] 1) Add distilled water to the algae powder according to the ratio of material to liquid 1:2.5, filter with suction, wash with deionized water for 3 times, add phosphate buffer with a pH of 7 and a concentration of 0.012mol / L and active polypeptide, algae powder and The weight-to-volume ratio of phosphate buffer solution is 1:14, stirred for 110 minutes, quickly frozen at -22°C, then thawed at 5°C, repeated freezing and thawing 4 times, high-speed refrigerated centrifugation, the supernatant is the crude protein solution, the activity Protein belongs to intracellular protein. First, the cell wall and cell membrane of the cell must be broken to dissolve it. The higher the degree of cell disruption, the higher the yield of active protein. The repeated freezing and thawing method utilizes the formation of intracellular ice particles and the increase of the salt concentration of the cel...

Embodiment 3

[0031] The specific steps of the preparation method of the active protein of Spirulina platensis are as follows: add distilled water to the algal powder according to the ratio of material to liquid of 1:2.8, suction filter, wash with deionized water for 2 to 3 times, add pH to 6.5, concentration is 0.01mol / L of phosphate buffer and active peptide, wherein the weight-volume ratio of algae powder to phosphate buffer is 1:14~16, the amount of active peptide added is 0.5% of the algae powder, stirred for 130min, and rapidly heated at -20°C Frozen, then thawed at 3°C, repeated freezing and thawing 3 times, and centrifuged; adding a concentration of 1.8wt% chitosan solution to the supernatant, the weight-to-volume ratio of algae powder and chitosan solution was 1:4, adjusted pH to 6.5, stirred for 25 minutes and then centrifuged, put the supernatant into an ice-water bath, added ammonium sulfate to make the concentration 53wt%, stirred until the ammonium sulfate was completely disso...

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Abstract

The invention discloses a method for preparing a spirulina platensis active protein. The method specifically comprises the following steps: putting algae powder into a phosphate buffer liquid and active polypeptides, and performing multigelation so as to obtain a crude protein liquid; adding ammonium sulfate into the crude protein liquid, and performing salting out; putting salted precipitate into a dialysis bag, and performing dialysis in the phosphate buffer liquid; performing a hydroxyapatite adsorption chromatography method on dialyzate, thereby obtaining a pure active protein product. The method has the beneficial effects that active proteins can be extracted by using the method, the extraction efficiency is improved, the yield of the active proteins is increased, the operation is simple, and no special instrument or equipment is needed; the active proteins prepared by using the method are high in purity, bright in color and rich in nutrition, capable of improving lymphocyte activity, improving body immunity through lymphatic systems, improving disease resistance of bodies, removing free radicals in living bodies and promoting hemocyte regeneration of animals, and has remarkable antitumor activity.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a preparation method of active protein from Spirulina platensis. Background technique [0002] Spirulina (Spirulina platensis) is a class of lower plants belonging to Cyanophyta and Oscillatoriaceae. Like bacteria, they do not have a real nucleus in their cells, so they are also called cyanobacteria. Spirulina is usually multicellular filamentous and cylindrical, loose or tight and regular spiral bends, but the shape is often changed by the growth environment, and some even produce stable linear variation. Spirulina likes high temperature (25-36°C) and high alkali (pH8~12). It is photosynthetic and autotrophic, but it can also use some organic matter in the environment for concurrent growth and asexual reproduction. The generation of compartments or the generation of pseudovacuoles in algal filaments forms algal growth stages. Spirulina contains a large amount of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/16
CPCC07K1/16C07K1/303C07K1/34C07K1/36
Inventor 江爱华
Owner 乐清瑞耀食品技术有限公司
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