Method constructed for verifying retina photoreceptor cell specific surface protein

Abstract

The invention relates to a method constructed for verifying retina photoreceptor cell specific surface protein. By utilizing the method provided by the invention, purified precursor cells of the photoreceptor cells are analyzed and screened to obtain the specific surface protein of the photoreceptor cells; the specific surface protein is specifically expressed on membrane surfaces of retina photoreceptor cells from human embryonic stem cells and human pluripotent stem cells, so that the induced human embryonic stem cells or the photoreceptor cells from the human pluripotent stem cells are purified by utilizing the specific surface protein. The purified photoreceptor cells are obtained through screening the surface protein and potential hazards caused by the fact that transgenosis is carried out or various small molecule compounds are used as an induction agent can be avoided. The key scientific problem is solved and a process of converting stem cells to clinical application is accelerated; requirements of medical researches are met and a foundation is laid for cell replacement therapy of human retina related diseases (such as glaucoma and macular eye diseases).

Description

technical field [0001] The invention relates to the field of biomedicine, relates to retinal cells, and in particular to a method for constructing and verifying specific surface proteins of retinal photoreceptor cells. Background technique [0002] The retina is a key structure that makes up the visual system. Clinically, there are effective treatments for refractive visual dysfunction, but there is still no solution for retinopathy, especially visual impairment or blindness caused by trauma, degeneration and other reasons. Since the retina is derived from the neuroectoderm, it has typical structural characteristics of the central nervous system, is sensitive to damage, and is difficult to regenerate, making it the crux of refractory eye diseases, and it is also the focus, difficulty, and area that needs to be overcome urgently in ophthalmology research. Over the years, many researchers at home and abroad have carried out extensive explorations for this purpose, and the Min...

AI Technical Summary

Problems solved by technology

The reasons are obvious. Firstly, when the differentiated cells are used for drug screening or functional identification in vitro, the reliability of the results will be affected by the mixing of other cells; secondly, if the mixed cells are transplanted into model animals, abnormalities will occur. Risk of hyperplasia or even tumorigenesis

Although many studies have been devoted to improving the induction and differentiation rate of ganglion cells, for example: transfecting Math5 into stem cells, and then obtaining purified ganglion cells through FACS screening, however, these methods have a fatal flaw, that is: transfecting the reporter vector Infection into stem cells will lead to changes in the stem cell genome, which limits its clinical application