Method constructed for verifying retina photoreceptor cell specific surface protein

A surface protein, photoreceptor cell technology, applied in the field of biomedicine, can solve problems such as abnormal proliferation, affect the reliability of results, tumorigenesis, etc., and achieve the effect of improving visual function

Pending Publication Date: 2017-11-14
上海三誉华夏基因科技有限公司
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AI Technical Summary

Problems solved by technology

The reasons are obvious. Firstly, when the differentiated cells are used for drug screening or functional identification in vitro, the reliability of the results will be affected by the mixing of other cells; secondly, if the mixed cells are transplanted into model animals, abnormalities will occur. Risk of hyperplasia or even tumorigenesis
Although many studies have been devo...

Method used

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Embodiment Construction

[0020] The "induced differentiation of human embryonic stem cells" described in the present invention adopts the method mentioned in the application number 201210301765.X, titled "A method for inducing the differentiation of human pluripotent stem cells into retinal precursor cells". Other unmentioned parts of the present invention are prior art.

[0021] Reporter cell line construction technology: The existing reporter cell line construction technologies include traditional zinc finger protein technology, Talen technology and Cas9 technology. We use Talen technology to construct reporter cell lines.

[0022] A method for constructing and verifying the specific surface protein of retinal photoreceptor cells, comprising the steps of:

[0023] (1) Using Talen technology, establish a human embryonic stem cell line of Crx-GFP, induce differentiation of this human embryonic stem cell line of Crx-GFP to the 6th week, and screen the photoreceptor cell precursor cells expressing GFP b...

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Abstract

The invention relates to a method constructed for verifying retina photoreceptor cell specific surface protein. By utilizing the method provided by the invention, purified precursor cells of the photoreceptor cells are analyzed and screened to obtain the specific surface protein of the photoreceptor cells; the specific surface protein is specifically expressed on membrane surfaces of retina photoreceptor cells from human embryonic stem cells and human pluripotent stem cells, so that the induced human embryonic stem cells or the photoreceptor cells from the human pluripotent stem cells are purified by utilizing the specific surface protein. The purified photoreceptor cells are obtained through screening the surface protein and potential hazards caused by the fact that transgenosis is carried out or various small molecule compounds are used as an induction agent can be avoided. The key scientific problem is solved and a process of converting stem cells to clinical application is accelerated; requirements of medical researches are met and a foundation is laid for cell replacement therapy of human retina related diseases (such as glaucoma and macular eye diseases).

Description

technical field [0001] The invention relates to the field of biomedicine, relates to retinal cells, and in particular to a method for constructing and verifying specific surface proteins of retinal photoreceptor cells. Background technique [0002] The retina is a key structure that makes up the visual system. Clinically, there are effective treatments for refractive visual dysfunction, but there is still no solution for retinopathy, especially visual impairment or blindness caused by trauma, degeneration and other reasons. Since the retina is derived from the neuroectoderm, it has typical structural characteristics of the central nervous system, is sensitive to damage, and is difficult to regenerate, making it the crux of refractory eye diseases, and it is also the focus, difficulty, and area that needs to be overcome urgently in ophthalmology research. Over the years, many researchers at home and abroad have carried out extensive explorations for this purpose, and the Min...

Claims

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Application Information

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IPC IPC(8): C12N5/079G01N33/68
CPCG01N33/68C12N5/0621G01N2333/47C12N2509/00
Inventor 王晓冰
Owner 上海三誉华夏基因科技有限公司
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