Application of aspirin and herceptin combination or synergy in oncotherapy

A technology of trastuzumab and aspirin, applied in the field of tumor treatment, can solve the problems of high cost and limit the clinical application of trastuzumab, and achieve the effect of increasing the curative effect

Active Publication Date: 2017-11-17
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although trastuzumab can effectively improve the efficacy of HER2-positive breast cancer and gastric cancer, it is regrettable that only about 30% of HER2-positive breast and gastric cancer patients have significant effects, and most of them initially received trastuzumab Patients who respond to anti-therapy often develop drug resistance within 1 year, coupled with its high cost, which greatly limits the clinical application of trastuzumab

Method used

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  • Application of aspirin and herceptin combination or synergy in oncotherapy
  • Application of aspirin and herceptin combination or synergy in oncotherapy
  • Application of aspirin and herceptin combination or synergy in oncotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This embodiment provides a drug combination for treating tumors, the drug combination comprising trastuzumab and aspirin for combined or co-administration with trastuzumab.

[0060] The drug combination provided in this embodiment has a good therapeutic effect on tumors, especially HER2-positive tumors such as HER2-positive breast cancer or HER2-positive gastric cancer.

Embodiment 2

[0062] 1 The effect of aspirin combined with trastuzumab on the proliferation of HER2-positive breast cancer cell SKBR3

[0063] MTT method to detect cell proliferation

[0064] The normal-growing SKBR3 cells were subcultured and seeded on a 96-well cell culture plate with a volume of 200μl per well and the number of cells was 2×10 3 After culturing overnight, add 5mM Aspirin (5mM Aspirin), 15μg / ml Trastuzumab (15μg / ml Trastuzumab), 30μg / ml Trastuzumab (30μg / ml Trastuzumab) and the combination of the two drugs (5mMAspirin). +15μg / ml Trastuzumab, that is, the mass ratio of aspirin to trastuzumab is 60:1), (5mMAspirin+30μg / ml Trastuzumab, that is, the mass ratio of aspirin to trastuzumab is 60:2) Continue to culture, and add an equal volume of water for injection or DMSO culture medium as the control group (ie control group), and detect cell proliferation after 2 days. Set the MTT configuration concentration to 5mg / ml, add 20μl to each well and continue to incubate for 4h, then disc...

Embodiment 3

[0071] The effect of aspirin combined with trastuzumab on the apoptosis of HER2-positive breast cancer cell SKBR3

[0072] Flow cytometry to detect cell apoptosis

[0073] After the normal growth of SKBR3 cells were subcultured, they were seeded in a culture dish. When the cell density reached about 80%, they were added containing 5mM aspirin (5mM Aspirin), 30μg / ml trastuzumab (30μg / ml Trastuzumab) and the combination of the two drugs. (5mM Aspirin+30μg / ml Trastuzumab, that is, the mass ratio of aspirin to trastuzumab is 60:2) The culture medium is continued to be cultured, and an equal volume of water for injection or DMSO medium is added as the control group (ie control group) , Treat the cells after 72h. Resuspend the cells after digestion and adjust the number of cells to 1×10 6 Pcs / ml, wash 2-3 times with PBS and add 500μl buffer to suspend the cells, then add 10μl Annexin V-FITC and 10μl PI to mix, and incubate at room temperature away from light After staining for 15 minut...

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Abstract

The invention discloses application of aspirin and herceptin combination or synergy in oncotherapy, and relates to the technical field of oncotherapy. Relative to alone using of herceptin, aspirin is combined with the herceptin for medication, proliferation of HER2 positive tumor cells can be obviously restrained, and apoptosis of the HER2 positive tumor cells is promoted; and effect of anti-HER2 positive tumor of aspirin and herceptin combination for medication is better than effect of alone using of the herceptin. According to the application of aspirin and herceptin combination or synergy in oncotherapy, new application of the aspirin is discovered for the first time, and the aspirin helps to increase curative effect of the herceptin; and through the application of aspirin and herceptin combination or synergy in preparation of medicines used for treating tumors, theoretical basis is not only provided for anticancer effect of the aspirin in HER2 positive tumors, meanwhile, but also a new idea and a treatment mean are provided for treatment of tumors, especially HER2 positive tumors such as HER2 positive breast cancer or HER2 positive gastric cancer.

Description

Technical field [0001] The present invention relates to the technical field of tumor treatment, in particular to the application of aspirin and trastuzumab in combination or synergy in tumor treatment. Background technique [0002] Currently, malignant tumors have become a major disease threatening human health. Human Epidermal Growth Factor Receptor-2 (Human Epidermal Growth Factor Receptor-2, Her-2)-positive malignant tumors are highly invasive, disease-free survival is short, and prognosis is poor. [0003] HER2-positive breast cancer accounts for 20%-30% of all breast cancers. Among all molecular types of breast cancer, the time to distant metastasis and the overall survival time of HER2-positive early breast cancer are relatively short. HER2 positive is a common molecular type of gastric cancer. According to statistics, about 20% of gastric cancers are HER2 positive, which is more malignant than other types of gastric cancer. Therefore, the treatment and research for HER2-po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K31/616A61P35/00
CPCA61K31/616A61K39/395A61K2300/00
Inventor 马骥张涛赵庆丽钟翠萍于晓辉吴伟强
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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