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A kind of production method of activity detection two-dimensional code

A production method and activity detection technology, which can be used in biological testing, material inspection products, and material analysis by observing the impact on chemical indicators. It has problems such as activity, and achieves the effect of low cost, improved practicability, and guaranteed confidentiality and effectiveness.

Inactive Publication Date: 2019-07-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to make up for the small amount of information carried by one-dimensional codes, poor confidentiality, and the lack of activity of traditional two-dimensional codes, the invention provides a method for making two-dimensional codes for activity detection with simple methods, accurate and effective load information, and detection results

Method used

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  • A kind of production method of activity detection two-dimensional code
  • A kind of production method of activity detection two-dimensional code
  • A kind of production method of activity detection two-dimensional code

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Prepare reagent solution: prepare Tris-Hcl buffer solution with a concentration of 0.1mol / L and a pH of 9.5, prepare 1mg / ml ALP solution with the buffer solution, and then dilute to 0.75mg / ml, 0.5mg / ml, 0.25mg / ml respectively ml, 0.1mg / ml, 0.05mg / ml ALP solution, prepare BCIP solution with a concentration of 50mg / ml with buffer, prepare NBT solution with a concentration of 50mg / ml with buffer, and mix the prepared BCIP / NBT solution Take 66μl and add it into 10ml Tris-Hcl buffer solution to make detection substrate solution.

Embodiment 2

[0049] Edit the content of the two-dimensional code information: Add the detection substrate solution and ALP solution of different concentrations on the filter paper respectively. After the reaction is complete and the color development is stable, use the scanner to obtain the color information, and use ImageJ to measure the gray value. In Excel Plot the characteristic curve and fit the characteristic equation. Edit the concentration and gray value comparison diagram and characteristic curve diagram into pictures and save them, such as figure 1 ; Then edit the detection content information, edit the picture and information through the online QR code generator and generate a QR code, such as figure 2 .

Embodiment 3

[0051] Pre-treat the detection substrate: soak the filter paper with a 0.75% bovine serum albumin solution, then dry the completely soaked filter paper in a blast oven at 37°C; use the generated two-dimensional code to make a stamp with a stamp machine, Use the stamp to pick up the detection substrate solution, and transfer the two-dimensional code to the filter paper soaked in the bovine serum albumin solution and dried.

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Abstract

The invention relates to a making method of an activity detection two-dimensional code, and belongs to the field of biochemical detection. The purpose of the invention is to provide the making method of the activity detection two-dimensional code, having the advantages of simplicity, information loading and accurate and effective detection result. The method comprises the following steps: editing content information, and using a computer two-dimensional code generator to make a two-dimensional code; preparing functional ink; using the prepared functional ink and a solution to be detected to generate a two-dimensional code on a detection substrate; and scanning the displayed two-dimensional code by a device after a reaction is complete and color development becomes stable in order to obtain detection information. The steps of the method are simple, the made activity detection two-dimensional code is displayed through the color development reaction of the functional ink and the solution to be detected and is identified by the device to obtain relevant information, the reaction can be completely carried out on a paper base, and the activity detection two-dimensional code can be widely applied to the field of biological detection and chemical detection.

Description

technical field [0001] The invention relates to a method for making a two-dimensional code for activity detection. The two-dimensional code is mainly displayed through the color reaction between functional ink and a detection solution and can be recognized by equipment to obtain relevant information, belonging to the field of biochemical detection. Background technique [0002] In the field of biochemical detection, colorimetry is widely used in the detection of enzymes, metal ions, proteins, nucleic acids, antibodies, DNA, pathogens, glucose, uric acid, nitrite ions and other disease markers, but in actual operation Among them, most of the test results are only displayed in the form of color blocks, and additional instructions are required to obtain relevant test information. Although some test results are presented in the form of letters, symbols, text or other patterns, the content of the load Very little, no more information can be provided to the user. [0003] To solv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52G01N21/78
CPCG01N21/78G01N33/52
Inventor 吴静田君飞徐军飞石聪灿
Owner SOUTH CHINA UNIV OF TECH
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