Preparation method and application of novel C19-diterpene alkaloid separated from Aconitum taronense

A technology of diterpene alkaloid, aconitum, applied in the field of phytochemistry

Inactive Publication Date: 2017-11-24
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the chemical components and pharmacological effects of Dulong Aconitum, so it is of great significance to conduct research on the anti-inflammatory chemical components of Dulong Aconitum

Method used

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  • Preparation method and application of novel C19-diterpene alkaloid separated from Aconitum taronense
  • Preparation method and application of novel C19-diterpene alkaloid separated from Aconitum taronense
  • Preparation method and application of novel C19-diterpene alkaloid separated from Aconitum taronense

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Embodiment Construction

[0020] A novel C isolated from Dulong Aconitum 19 - diterpene alkaloid taronenine A, structural formula:

[0021]

[0022] The preparation method of a novel diterpene alkaloid in the root of Dulong Aconitum adopts the following steps:

[0023] Step 1: Take 6.0kg of dried roots of Aconitum taronense Fletcher et Lauener and crush them;

[0024] Step 2: Use 50 L of 0.5% hydrochloric acid for percolation until there is no alkaloid reaction in the percolation liquid, then basify with 10% ammonia water to pH 9, extract with an equal volume of ethyl acetate, extract three times, combine ethyl acetate, Concentrated under reduced pressure and sucked dry to obtain 41.5g of total alkaloid extract;

[0025] Step 3: The total alkali extract is eluted with a gradient of 100-200 mesh through silica gel column chromatography 氯仿 :V 甲醇 , 100:1→1:1, TLC detection merged to obtain nine parts of FrA-FrI, wherein FrD was 9.0g and eluted by silica gel column chromatography 300-400 mesh gradie...

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Abstract

The invention discloses a preparation method and application of a novel C19-diterpene alkaloid separated from buttercup Aconitum plant Aconitum taronense. By using the root of Aconitum taronense as a raw material, the novel C19-diterpene alkaloid taronenine A is separated and identified by using an acid water penetrating method, a solvent extraction method, column chromatography on silica gel and a spectroscopy method, in vitro anti-inflammatory experiments indicate that the compound has an obvious effect for inhibiting lipopolysaccharide mediated mouse macrophage RAW 264.7 from releasing skin reaction factor interleukin-6, and the foundation is established for developing novel anti-inflammatory drugs from the plants.

Description

technical field [0001] The invention relates to the field of phytochemistry, in particular to a novel C isolated from the root of Aconitum aconitum 19 - Preparation method and use of diterpene alkaloids. Background technique [0002] Aconitum (Aconitum) is an annual or perennial herb of the Ranunculaceae (Ranunculaceae). This is a plant. Most of the plants in this genus have analgesic, rheumatism and antipyretic effects. Diterpene alkaloids are the characteristic active ingredients of this genus of plants, which have significant effects in anti-inflammation, analgesia, and anti-arrhythmia. At present, the diterpene alkaloids that have been developed and used for clinical medicine include aconitine, 3-acetylaconitine, aconitin and guanfu A, among which aconitin can also be used to treat rheumatoid arthritis, Inflammation such as frozen shoulder and osteoarthritis. Therefore, it is very possible to find anti-inflammatory drugs from Aconitum plants. [0003] Dulong aconit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/08A61P29/00
CPCC07D471/08
Inventor 尹田鹏陈阳蔡乐胡晓芳
Owner ZUNYI MEDICAL UNIVERSITY
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