Kit for detecting mutation of gastrointestinal stromal tumor-related genes and application thereof

A gastrointestinal stromal tumor and kit technology, applied in the field of biomedicine, can solve the problems of long detection cycle, low sensitivity, and unsuitability

Active Publication Date: 2017-12-01
上海讯迈丰生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection samples of this technology are aimed at tumor tissues or slices. The sample collection is inconvenient, the detection cycle is long, and due to its own l...

Method used

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  • Kit for detecting mutation of gastrointestinal stromal tumor-related genes and application thereof
  • Kit for detecting mutation of gastrointestinal stromal tumor-related genes and application thereof
  • Kit for detecting mutation of gastrointestinal stromal tumor-related genes and application thereof

Examples

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Embodiment 1

[0056] Example 1. Establishment and application of the method for detecting gastrointestinal stromal tumor-related gene mutations according to the present invention

[0057] 1. Samples for testing

[0058] Plasma samples (date received: 2015 / 11 / 17) from a patient (female, 67 years old) who was clinically diagnosed as GIST (high risk) in the General Hospital of the Chinese People's Liberation Army, numbered P151119N00101 (patients informed and consented). Plasma free DNA was extracted with Kangwei Century Free DNA Extraction Kit CW2603S and set aside.

[0059] 2. Detection of gene mutations related to gastrointestinal stromal tumors in the samples to be tested

[0060] The general process of the detection of gastrointestinal stromal tumor-related gene mutations in the sample to be tested provided by the present invention is as follows: (1) Step 1 PCR amplification, amplifying the target gene fragment; (2) agarose gel electrophoresis, and magnetic beads Purification and recove...

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Abstract

The invention discloses a kit for detecting the mutation of gastrointestinal stromal tumor-related genes and application thereof. The invention provides application of a primer pair group (as shown in sequence 1 to sequence 40) and a readable vector recording a method for detecting the mutation of the gastrointestinal stromal tumor-related genes of a to-be-detected person to preparation of the kit used for detecting the mutation of the gastrointestinal stromal tumor-related genes. According to the invention, instead of traditional tumor tissue or section, blood plasma is use as a detection sample in the invention; 20 specific primer pairs are designed directed at the gastrointestinal stromal tumor-related genes, i.e., a KIT gene and a PDGFRA gene; a high-flux sequencing detection method is employed; so the data volume of detection is increased, the accuracy of experimental results is improved, and the mutation of the gastrointestinal stromal tumor-related genes can be accurately and effectively monitored.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a kit for detecting gene mutations related to gastrointestinal stromal tumors and its application. Background technique [0002] Gastrointestinal stromal tumor (GIST) is a rare tumor of the gastrointestinal tract, which mostly occurs in the muscular layer of the gastrointestinal tract, and is also called leiomyoma or leiomyosarcoma. As early as 1983, Mazur et al proposed the concept of GIST based on electron microscope observation and immunohistochemical detection results. GIST originates from Cajal interstitial cells, most of which have proto-oncogene c-kit mutations, and platelet-derived growth factor receptor (PDGFR) α gene mutations can be found in some without c-kit gene mutations. Therefore, genetic analysis has applications in clinical GIST diagnosis. The detection of gene mutation type shows that more than 80% of the gene mutation types of gastrointestinal stromal tumors are KI...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2535/122C12Q2537/143C12Q2545/113
Inventor 伍建姬晓雯李巧玲
Owner 上海讯迈丰生物科技有限责任公司
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