Method for rapidly detecting content of galantamine in amaryllidaceae plants

Abstract

A method for rapidly detecting content of galantamine in amaryllidaceae plants comprises the steps that based on a liquid chromatography-mass spectrometry technology, isocratic elution is carried out, liquid chromatography is in series connection with secondary mass spectrometry, analysis is performed through a Full MS / dd-MS2 mode or an MRM mode under positive ion scanning, alkaloids in the amaryllidaceae plants, such as galantamine, can be quickly extracted and accurately qualitatively analyzed, the analysis time is significantly reduced, the accuracy of analysis is improved, a detection result can be obtained in a program time within 3.0 min, the retention time of galantamine is within 1 min, the recovery rate is 95.7-97.1%, and a relative standard deviation is 1.3-2.7%. No other characteristic peak interference occurs, and the degree of resolution is complete separation, the analysis speed and accuracy are greatly enhanced, and the effective and accurate analytical method is provided for studying the accumulation law and anabolic metabolism regulation of galantamine in the amaryllidaceae plants.

Description

technical field [0001] The invention belongs to the field of detection of alkaloid substances, in particular to a method for rapidly detecting the content of galantamine in Lycoris. Background technique [0002] Amaryllidaceae (Lycoris spp.) and Hippestrum spp. are bulbous herbs of the family Amaryllidaceae. Many species are unique ornamental and medicinal flowers in China. They are rich in alkaloid components unique to Amaryllidaceae plants . [0003] So far, more than 500 alkaloid compounds with different structures have been isolated from Amaryllidaceae plants. Phytochemical studies have shown that these compounds have a variety of pharmacological functions and have high medicinal value. Wherein, galantamine is a kind of isoquinoline alkaloid, as acetylcholinesterase inhibitor, can affect brain nicotine receptor and inhibit acetylcholinesterase activity, is the treatment Alzheimer's disease (Alzheimer's disease, senile dementia early stage. Symptoms), the drug has been ...

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Problems solved by technology

[0009] Sahar et al. (Sahar et al., 4'-O-Methylnorbelladine feeding enhances galanthamine and lycorine production by Leucojum aestivum L.shoot cultures, Engineering in Life Sciences, 00: 1-6, 2015) adopted the method of HPLC-ESI-MS, for Quantitative analysis of galantamine and lycorine in lotus buds of Xia Xuepian, using gradient elution, requires a long analysis time in the early and late stage of gradient elution, the detection program takes 36 minutes, and the elution time of galantamine The HPLC analysis time is relatively long, about 36 minutes, and the content detection is carried out according to the retention time and precursor ion information. Compared with the method of Quan et al., the accuracy of this method is improved, but the obvious defect is that the same elution There may be many kinds of material components of time and precursor ion size, so there are still defects in qualitative and quantitative accuracy

[0010] Moreover, in the prior art, there are also problems of long extraction time and cumbersome operation. For example, in the method of Quan et al., the main extraction steps are drying, filtration, methanol extraction for 3 hours, filtration, evaporation, pH adjustment, and chloroform extraction for 3 times. , evaporation, methanol reconstitution, filtration

In the method of Sahar et al. (Sahar et al., 2015), the main extraction steps are: methanol extraction for 24 hours, concentration, sulfuric acid acidification, chloroform extraction, evaporation, reconstitution, and filtration, all of which are rapid quantitative analysis bands for galantamine. Inconvenience

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