Method for rapidly detecting content of galantamine in amaryllidaceae plants

A technology of galantamine and Amaryllidaceae, applied in the field of rapid detection of galantamine content in Lycoris, can solve the problems of qualitative and quantitative accuracy defects, inconvenience of rapid quantitative analysis, long analysis time, etc., and save plant materials and extraction reagents, improve analysis speed and accuracy, and ensure the effect of extraction

Inactive Publication Date: 2017-12-12
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0009] Sahar et al. (Sahar et al., 4'-O-Methylnorbelladine feeding enhances galanthamine and lycorine production by Leucojum aestivum L.shoot cultures, Engineering in Life Sciences, 00: 1-6, 2015) adopted the method of HPLC-ESI-MS, for Quantitative analysis of galantamine and lycorine in lotus buds of Xia Xuepian, using gradient elution, requires a long analysis time in the early and late stage of gradient elution, the detection program takes 36 minutes, and the elution time of galantamine The HPLC analysis time is relatively long, about 36 minutes, and the content detection is carried out according to the retention time and precursor ion information. Compared with the method of Quan et al., the accuracy of this method is improved, but the obvious defect is that the same elution There may be m

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  • Method for rapidly detecting content of galantamine in amaryllidaceae plants
  • Method for rapidly detecting content of galantamine in amaryllidaceae plants
  • Method for rapidly detecting content of galantamine in amaryllidaceae plants

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Embodiment 1

[0054] Embodiment 1 A method for rapidly detecting galantamine content in Lycoris, comprising the steps of:

[0055] 1) Draw a standard curve

[0056] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C 17 h 21 NO 3 , molecular weight 287.359), dissolved with methanol and fixed to 100ml to obtain its standard stock solution, the concentration of the stock solution is 100 μg / mL;

[0057] Standard intermediate solution: Accurately pipette 1mL standard stock solution into a 100mL volumetric flask, and use methanol to make up a mixed standard intermediate solution of 1.0μg / mL;

[0058] Standard working solution: pipette an appropriate amount of mixed standard intermediate solution as needed, and dilute with methanol to form standard working solutions with concentrations of 2ng / mL, 6ng / mL, 20ng / mL, 60ng / mL and 100ng / mL.

[0059] Utilize the chromatograph connected in series with the secondary mass spectrometer to measure the gal...

Embodiment 2

[0099] Embodiment 2 A method for rapidly detecting galantamine content in Hippeastrum, comprising the following steps

[0100] 1) Draw a standard curve

[0101] Accurately weigh an appropriate amount of galantamine standard substance 0.010g (Gal, Galanthamine, C17H21NO3, molecular weight 287.359) with an analytical balance, dissolve it with methanol and set the volume to 100ml to obtain its standard stock solution, and the concentration of the stock solution is 100 μg / mL; Standard intermediate solution: Accurately pipette 1mL standard stock solution into a 100mL volumetric flask, and use methanol to make up a 1.0μg / mL mixed standard intermediate solution; standard working solution: pipette an appropriate amount of mixed standard intermediate solution as needed, and use Methanol was diluted into standard working solutions with concentrations of 0.5ng / mL, 1ng / mL, 5ng / mL, 10ng / mL, 50ng / mL, and 100ng / mL.

[0102] The galantamine standard solution is determined by a chromatograph ...

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Abstract

A method for rapidly detecting content of galantamine in amaryllidaceae plants comprises the steps that based on a liquid chromatography-mass spectrometry technology, isocratic elution is carried out, liquid chromatography is in series connection with secondary mass spectrometry, analysis is performed through a Full MS/dd-MS2 mode or an MRM mode under positive ion scanning, alkaloids in the amaryllidaceae plants, such as galantamine, can be quickly extracted and accurately qualitatively analyzed, the analysis time is significantly reduced, the accuracy of analysis is improved, a detection result can be obtained in a program time within 3.0 min, the retention time of galantamine is within 1 min, the recovery rate is 95.7-97.1%, and a relative standard deviation is 1.3-2.7%. No other characteristic peak interference occurs, and the degree of resolution is complete separation, the analysis speed and accuracy are greatly enhanced, and the effective and accurate analytical method is provided for studying the accumulation law and anabolic metabolism regulation of galantamine in the amaryllidaceae plants.

Description

technical field [0001] The invention belongs to the field of detection of alkaloid substances, in particular to a method for rapidly detecting the content of galantamine in Lycoris. Background technique [0002] Amaryllidaceae (Lycoris spp.) and Hippestrum spp. are bulbous herbs of the family Amaryllidaceae. Many species are unique ornamental and medicinal flowers in China. They are rich in alkaloid components unique to Amaryllidaceae plants . [0003] So far, more than 500 alkaloid compounds with different structures have been isolated from Amaryllidaceae plants. Phytochemical studies have shown that these compounds have a variety of pharmacological functions and have high medicinal value. Wherein, galantamine is a kind of isoquinoline alkaloid, as acetylcholinesterase inhibitor, can affect brain nicotine receptor and inhibit acetylcholinesterase activity, is the treatment Alzheimer's disease (Alzheimer's disease, senile dementia early stage. Symptoms), the drug has been ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 李青竹蔡友铭张永春杨柳燕李心
Owner SHANGHAI ACAD OF AGRI SCI
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