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A kind of method for rapidly growing seedlings of dendrobium candidum asexual propagation

A technique for asexual reproduction of Dendrobium candidum, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of unfavorable conservation and propagation of fine varieties of Dendrobium candidum, difficulty in realizing large-scale reproduction of Dendrobium candidum, and callus growth Slow and other problems, to achieve the effect of saving manpower and raw materials, saving seedling cost, and low cost

Active Publication Date: 2019-10-08
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the foreign language magazine "In vitro Cellular&Developmental Biology--Plant" (2008, 4 volumes 3 phases), it is called "Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall exLindl" and "Zhao, P. ,Wu,F.,Feng,F.S.S.,Wang,W.J.J.,2008.Protocorm-like body(PLB)formation and plant regeneration from the callus culture of Dendrobiumcandidum Wall ex Lindl.In Vitro Cellular&Developmental Biology:Plant 44,178–185." It is reported in the paper that the callus grows slowly and is easy to brown during the tissue culture process of Dendrobium nobile, and it is difficult to achieve large-scale propagation of Dendrobium officinale
In the prior art, the patent with the announcement number of 102499092 discloses a method for the propagation and cultivation of dendrobium cell tissue. This method requires multiple transfer cultures to prepare a number of culture media with different formulations, and the culture period is long, which is difficult to pass. This culture method obtains corresponding economic benefits, which is not conducive to the conservation and propagation of fine varieties of Dendrobium officinale

Method used

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  • A kind of method for rapidly growing seedlings of dendrobium candidum asexual propagation
  • A kind of method for rapidly growing seedlings of dendrobium candidum asexual propagation
  • A kind of method for rapidly growing seedlings of dendrobium candidum asexual propagation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1), plant screening: select the Dendrobium officinale plant of 1-2 years of growth period, take the stem tip with a stem node without leaves, and the length of the stem tip is 0.5-1cm, or the length is 0.5-1cm. Stem segments from one node were used as explants.

[0023] 2), preparation of culture medium: use improved MS, that is, MS with double calcium ions added as the basic medium, and add NAA 1.5mg / L, 6-BA 0.5mg / L, agar powder 6g / L, Sucrose 30g / L, activated carbon 2g / L and potato juice 200g / L, adjust the pH value to 5.8, and sterilize at 121°C and 102.9kPa for 30min.

[0024] 3), inoculation of explants: place the stem tips and stem segments in step 1) in the culture medium, and cultivate them in the tissue culture room for 20 to 40 days at a culture temperature of 25°C, irradiated by fluorescent lamps, with a photoperiod of 12h, and light The intensity is 2500lx.

[0025] Observe and record the growth situation of shoot tip and stem section on the medium; the form...

Embodiment 2

[0027] To prepare the potato juice:

[0028] Weigh the potatoes according to the proportion (200g potatoes per 1L medium), clean the potatoes, remove the skin, cut into small pieces of 1-1.5cm square, put the potato pieces in boiling distilled water, cook for 20min, and then use 0.1% Sterilized with mercuric litre and 75% alcohol. Use three layers of gauze to filter the boiled potatoes and distilled water, and collect the filtrate, which is potato juice.

Embodiment 3

[0030] 1), respectively take the stem tip with a stem node in the growth period of 1-2 years, without leaves, and the length of the stem tip is 0.5-1cm, or the stem segment with a length of 0.5-1cm and a stem segment as explants.

[0031] 2), prepare culture medium according to the addition ratio in Table 1, except the composition in Table 1, also include agar powder 6g / L, sucrose 30g / L, gac 2g / L and potato juice 200g / L in the culture medium.

[0032] Add different concentrations of hormone ratio in the medium in Table 1

[0033] Medium number basic medium NAA(mg / L) 6-BA(mg / L) 2,4-D (mg / L) 1 MS 2.0 0.5 0 2 MS 1.5 0.5 0 3 MS 1.0 0.5 0 4 MS 0.5 0.5 1.5 5 MS 1.0 0.5 1.5 6 MS 0.5 0.5 2.0 7 MS 0.4 1.0 5.0 8 MS 0.2 0.5 2.5 9 Modified MS (2 times Ca 2+ )

3.0 1.0 0 10 Modified MS (2 times Ca 2+ )

1.5 0.5 0

[0034] 3), the shoot tip and the stem section are respecti...

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Abstract

The invention relates to the technical field of dendrobe vegetative propagation. The vegetative propagation method rapid seedling formation method for dendrobium officinale is short in seedling growing period, high in seedling formation rate and simple in operation process. By adopting the technical scheme, by adopting plant screening, stem tips or a stem with a stem node is taken from the dendrobium officinale wth the robust growing vigor as an explant; a culture medium is prepared, MS or improved MS is adopted as the basic culture medium, NAA, 6-BA, 2,4-D, agar powder, saccharose, activated carbon and potato juice are added, the pH value is adjusted to 5.8, and sterilization is performed for 30 min on the conditions that the temperature is 121 DEG C and the pressure is 102.9 kPa; the explant is inoculated to the culture medium, and the complete seedling plant is cultured from the explant directly. The seedling period is short, the cost is low, the operation process is short, the cultured seedling formation rate is high, and the merit of the mother plant can be kept.

Description

technical field [0001] The invention belongs to the technical field of asexual propagation of dendrobium nobile, in particular to a method for rapidly growing seedlings through asexual propagation of dendrobium officinale. Background technique [0002] Dendrobium is a kind of medicinal plant. It tastes sweet, slightly salty, and cold in nature. It returns to the stomach, kidney, and lung meridians. Modern pharmacological studies have proved that dendrobine contained in dendrobium is a good antipyretic and analgesic, and has the effects of enhancing immunity, delaying aging, and anti-tumor. Dendrobium not only has extremely high medicinal value, but also has certain ornamental value, occupying an important position in the international flower market. [0003] my country is rich in resources of Dendrobium candidum, mainly in the wild. However, due to long-term unrestrained exploitation and destruction by humans, as well as continuous changes in the natural environment, wild D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡亚东淳泽张雪琴刘静赵若茜郑世刚
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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