Primer for screening reference genes of syzygium samarangense under low-temperature stress and application thereof

Abstract

The invention provides a primer for screening reference genes of syzygium samarangense under low-temperature stress and application thereof. The primer comprises forward primer 5'-CCTGGCCTCTATGATCTTATCC-3' and reverse primer 5'-CAGGCATCATGTGCTTTG-3'. The expression level of 7 candidate reference genes ACT-7, GAPDH, UBQ, alpha-TUB, CYP20-1, EF-2 and APRT-5 under fruit development and low-temperature stress of syzygium samarangense can be detected by utilizing qRT-PCR, and gene stability is evaluated through geNorm, NormFinder, RestKeeper and RefFinder software. Results indicate that the reference gene CYP20-1 is expressed most stably under low-temperature stress, so that the situation that no reference gene exists in existing syzygium samarangense quantitative PCR detection can be solved, and foundation is laid for subsequent gene expression work.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer used for screening an internal reference gene under low temperature stress of the lotus mist and its application. Background technique [0002] wax apple〔 Syzygium. samarangense (BI.) Merr.et Perry] is the genus of Mytaceae (Mytaceae) Syzygium ) evergreen tree, is a typical tropical fruit tree. Lianwu fruit is rich in nutrients, has antidiarrheal, analgesic, antibacterial, anti-inflammatory (Tina et al. 2011) and other effects, and is bright in color, crisp and refreshing, and is loved by consumers. It is developing rapidly in suitable planting areas in my country and is an important tropical fruit. kind. In terms of production, unstable fruit quality and low temperature and cold damage in winter are two prominent problems hindering the development of the lotus fog industry, which need to be solved urgently. For a long time, optimizing cultivation measures a...

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening of internal reference genes of lotus mist. The present invention selects 7 commonly used internal reference genes, and uses qRT-PCR technology to evaluate their expression stability under different experimental conditions, in order to screen out stable expression genes under low temperature stress. Internal reference genes, laying the foundation for subsequent gene expression work

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