Multi-channel microfluidic chip apparatus for simultaneous detection of a variety of subtype swine influenzas

Abstract

The invention relates to a multi-channel microfluidic chip device for simultaneously detecting multiple subtypes of swine influenza, belonging to the field of analysis and testing. There are many obstacles in using cheap and easy-to-process polydimethylsiloxane (PDMS) to fabricate the substrate of microfluidic chip for subtype swine flu diagnosis; the surface of PDMS is strongly hydrophobic, and the effect of surface chemical modification is difficult to last. The main point of this case is that the substrate material is PDMS with an original surface, and the channels of the chip are filled with doped micron-sized titanium dioxide particles; the titanium dioxide particles are titanium dioxide that can induce super-hydrophilic properties on its surface through prior visible light irradiation Particles; the collection of super-hydrophilic channels between the particles of the titanium dioxide particle filler compensates and surpasses the original strong hydrophobic channel, thereby achieving a constant and super-hydrophilic modification effect; the titanium dioxide filler The function also includes preventing the collapse and deformation of the pipe of the chip, and preventing biological macromolecules from contacting the inner wall of the pipe.

Description

technical field [0001] The invention relates to a multi-channel microfluidic chip device for simultaneously detecting multiple subtypes of swine influenza, belonging to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic subtype swine influenza diagnosis, please refer to CN 201110311127.1 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about the key issues of this case. [0005] The basic structure of a microfluidic chip includes a substrate etched with channels ...

AI Technical Summary

Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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