Method for quickly and accurately authenticating tomato seedling stage heat resistance

A technology for tomato seedlings and heat resistance, applied in the field of identifying heat resistance of tomato seedlings

Inactive Publication Date: 2018-01-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for quickly, accurately and non-destructively identifying the heat tolerance of tomato seedli

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for quickly and accurately identifying heat tolerance of tomato seedlings:

[0024] (1) Put the matrix evenly into the hole tray and water thoroughly; sow the tomato seeds in the hole tray equipped with the matrix, sow 1-2 seeds in each hole, and cover with a layer of matrix after sowing. The matrix is ​​formed by mixing peat, vermiculite and perlite in a weight ratio of 1:1:1. Cultivate under suitable conditions such as outside temperature not higher than 30°C and light, water and humidity.

[0025] (2) 22 days after sowing, transplant the seedlings into a nutrient pot containing the same substrate, place the nutrient pot in an iron plate, and pour 600mL of Japanese garden test formula nutrient solution into the iron plate every day. The height of the nutrition bowl is 9cm, and the diameter is 11cm. The length of iron dish is 50cm, and width is 50cm, and height is 2cm, and every iron dish places 15 strains of seedlings.

[0026] (3) After 25 days of sowing,...

Embodiment 2

[0033] Using the method of Example 1, the heat tolerance of wild gooseberry tomato (LA1994) and conventional tomato varieties (Emperor Yellow Cherry) was identified and compared. Using Handy PEA, 4 plants of each variety were assayed. Mean F of wild gooseberry tomato and conventional tomato varieties before heat treatment v / F m 0.816 and 0.818, respectively, the mean F of wild gooseberry tomato and conventional tomato varieties after high temperature treatment v / F m They are 0.806 and 0.742, respectively. F of leaves of wild gooseberry tomato after high temperature treatment v / F m Significantly higher than conventional tomato varieties, and greater than 0.8; F of conventional tomato varieties leaves after high temperature treatment v / F m Less than 0.8 and significantly lower than before high temperature treatment. Therefore, wild gooseberry tomatoes are heat tolerant, while conventional tomato varieties are sensitive to high temperatures.

[0034] In order to high...

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Abstract

The invention relates to the technical field of tomato resistance authentication organisms, in particular to a method for quickly, accurately and non-destructively authenticating tomato seedling stageheat resistance. Tomato seeds which need to be authenticated are sown into a plug loaded with a medium, and cultivation is conducted on the condition that the external temperature is not higher than30 DEG C and illumination, moisture, humidity and the like are appropriate. After sowing is conducted for 22 days, seedlings are transplanted into a nutrient pot with the same medium, and the seedlings are watered with a Japanese garden test composition nutrient solution every morning. After sowing is conducted for 25 days, the seedlings are transplanted to an artificial climate tank. After adapting is conducted for 3 days, high temperature treatment is started. Firstly, the seedlings are pretreated for 10 h at 28 DEG C under the dark condition and then are subjected to the high temperature treatment for 12 h at 40 DEG C under the illumination condition; next, a leaf chamber clamp is utilized to clamp the first, the second and the third complete spread leaves of each plant, when dark adapting is conducted for 20 min, Handy PEA is utilized to measure Fv/Fm, and average Fv/Fm of each plant is calculated.

Description

technical field [0001] The invention relates to the technical field of tomato resistance identification biotechnology, specifically a method for identifying heat resistance of tomato seedlings. Background technique [0002] Tomato (Solanum lycopersicum L.), native to the Andes in South America, is widely used in open field and protected cultivation, and is one of the most important vegetable crops in the world. In the past 100 years, the global average temperature has increased by 0.5°C; by 2100, the global average temperature is expected to increase by 1°C–3.4°C. With global warming, high temperature stress has become a serious problem faced by global agriculture, and its impact on tomato production is becoming more and more serious. In the oversummer cultivation of tomato, high temperature also often occurs. High temperature stress can cause reversible or irreversible damage to the growth and development of tomato. When the ambient temperature exceeds 35°C, tomato seed ...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G9/16A01G31/00
CPCY02A40/25
Inventor 周蓉赵统敏徐丽萍赵丽萍王银磊余文贵宋刘霞
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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